Mouse Nbl1 ELISA Kit

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  • Alternative name

    Mouse N03 ELISA Kit;Mouse zinc finger protein DAN ELISA Kit;Mouse DAN ELISA Kit;Mouse Dana ELISA Kit;Mouse D4H1S1733E ELISA Kit;Mouse NO3 ELISA Kit;Mouse neuroblastoma, suppression of tumorigenicity 1 ELISA Kit;Mouse neuroblastoma suppressor of tumorigenicity 1 ELISA Kit;

  • Catalog
    E050545
  • species
    Mouse
  • GeneNbl1
  • Standard CurveMouse Nbl1 ELISA Kit
  • Other Species Human NBL1 ELISA KitChicken NBL1 ELISA KitRat Nbl1 ELISA Kit
  • SpecificityNatural and recombinant mouse DAN
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity Sample For quantitative detection of Mouse DAN in cell culture supernates, serum and plasma
  • Intended UseMouse Nbl1 ELISA Kit allows for the in vitro quantitative determination of Nbl1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: Mouse DAN ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for DAN has been precoated onto 96-well plates. Standards (NSO, A17-D178) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DAN is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse DAN amount of sample captured in plate. Background: Differential screening-selected gene aberrative in neuroblastoma (DAN) is a member of the DAN family of secreted glycoproteins that are putative BMP antagonists. The NBL1 gene, also known as DAN, is originally cloned from a normal rat fibroblast cDNA library by a differential screening method. The human DAN gene is mapped to chromosome 1p36.13-p36. It is found that the DAN gene possesses a tumor suppressive activity when overexpressed in v-src transformed cells.
  • Mouse Neuroblastoma suppressor of tumorigenicity 1 Protein information
  • Uniprot ID NBL1_MOUSE
  • Uniprot AC GKEPSHEG LNVYVQGEDS PGSQPGPHSH AHPHPGGQTP EPEEPPGAPQ VEEEGAED
  • UniGene Mm.9404;
  • GeneID 17965
  • KEGG mmu:17965;
  • Mouse Neuroblastoma suppressor of tumorigenicity 1 Protein SEQUENCE
  • SEQUENCE 178 AA; 19107 MW; C51D74C82ED904DD CRC64;

    MLWVLVGAVL PVMLLAAPPP INKLALFPDK SAWCEAKNIT QIVGHSGCEA

    KSIQNRACLG QCFSYSVPNT FPQSTESLVH CDSCMPAQSM WEIVTLECPG

    HEEVPRVDKL VEKIVHCSCQ ACGKEPSHEG LNVYVQGEDS PGSQPGPHSH

    AHPHPGGQTP EPEEPPGAPQ VEEEGAED

  • UCSC uc008vls.1; mouse.;


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