Human ITGA5 ELISA Kit

Alternative name
Human CD49 antigen-like family member E ELISA Kit;Human fibronectin receptor subunit alpha ELISA Kit;Human integrin alpha-F ELISA Kit;Human VLA-5 ELISA Kit;Human FNRA ELISA Kit;Human CD49e ELISA Kit;Human VLA5A ELISA Kit;Human integrin subunit alpha 5 ELISA Kit;Human integrin alpha-5 ELISA Kit;Human fibronectin receptor, alpha polypeptide ELISA Kit;Human fibronectin receptor, alpha subunit ELISA Kit;Human integrin, alpha 5 (fibronectin receptor, alpha polypeptide) ELISA Kit;Human very late activation protein 5, alpha subunit ELISA Kit;
Catalog
E020084
species
Human
GeneITGA5
SpecificityThis assay has high sensitivity and excellent specificity for detection of Integrin Alpha 5 (ITGa5). No significant cross-reactivity or interference between Integrin Alpha 5 (ITGa5) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.062ng/mL.
Intended UseHuman ITGA5 ELISA Kit allows for the in vitro quantitative determination of ITGA5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Alpha 5 (ITGa5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Integrin Alpha 5 (ITGa5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Integrin Alpha 5 (ITGa5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Integrin Alpha 5 (ITGa5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Integrin alpha-5 light chain Protein information
Uniprot ID ITA5_HUMAN
Uniprot AC P08648; Q96HA5;
UniGene Hs.505654;
GeneID 3678
KEGG hsa:3678;
Human Integrin alpha-5 light chain Protein SEQUENCE
SEQUENCE 1049 AA; 114536 MW; 6B4D558D4F739CBA CRC64;
MGSRTPESPL HAVQLRWGPR RRPPLLPLLL LLLPPPPRVG GFNLDAEAPA
VLSGPPGSFF GFSVEFYRPG TDGVSVLVGA PKANTSQPGV LQGGAVYLCP
WGASPTQCTP IEFDSKGSRL LESSLSSSEG EEPVEYKSLQ WFGATVRAHG
SSILACAPLY SWRTEKEPLS DPVGTCYLST DNFTRILEYA PCRSDFSWAA
GQGYCQGGFS AEFTKTGRVV LGGPGSYFWQ GQILSATQEQ IAESYYPEYL
INLVQGQLQT RQASSIYDDS YLGYSVAVGE FSGDDTEDFV AGVPKGNLTY
GYVTILNGSD IRSLYNFSGE QMASYFGYAV AATDVNGDGL DDLLVGAPLL
MDRTPDGRPQ EVGRVYVYLQ HPAGIEPTPT LTLTGHDEFG RFGSSLTPLG
DLDQDGYNDV AIGAPFGGET QQGVVFVFPG GPGGLGSKPS QVLQPLWAAS
HTPDFFGSAL RGGRDLDGNG YPDLIVGSFG VDKAVVYRGR PIVSASASLT
IFPAMFNPEE RSCSLEGNPV ACINLSFCLN ASGKHVADSI GFTVELQLDW
QKQKGGVRRA LFLASRQATL TQTLLIQNGA REDCREMKIY LRNESEFRDK
LSPIHIALNF SLDPQAPVDS HGLRPALHYQ SKSRIEDKAQ ILLDCGEDNI
CVPDLQLEVF GEQNHVYLGD KNALNLTFHA QNVGEGGAYE AELRVTAPPE
AEYSGLVRHP GNFSSLSCDY FAVNQSRLLV CDLGNPMKAG ASLWGGLRFT
VPHLRDTKKT IQFDFQILSK NLNNSQSDVV SFRLSVEAQA QVTLNGVSKP
EAVLFPVSDW HPRDQPQKEE DLGPAVHHVY ELINQGPSSI SQGVLELSCP
QALEGQQLLY VTRVTGLNCT TNHPINPKGL ELDPEGSLHH QQKREAPSRS
SASSGPQILK CPEAECFRLR CELGPLHQQE SQSLQLHFRV WAKTFLQREH
QPFSLQCEAV YKALKMPYRI LPRQLPQKER QVATAVQWTK AEGSYGVPLW
IIILAILFGL LLLGLLIYIL YKLGFFKRSL PYGTAMEKAQ LKPPATSDA
UCSC uc001sga.4; human.;

Category

Human ITGA5 ELISA Kit