SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse CDKN1A. No significant cross-reactivity or interference between Mouse CDKN1A and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse CDKN1A and all the analogues, therefore, cross reaction may still exist.
Intended UseMouse Cdkn1a ELISA Kit allows for the in vitro quantitative determination of Cdkn1a , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CDKN1A in Mouse serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CDKN1A. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse CDKN1A and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CDKN1A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse CDKN1A. You can calculate the concentration of Mouse CDKN1A in the samples by comparing the OD of the samples to the standard curve.
Mouse Cyclin-dependent kinase inhibitor 1 Protein information
Uniprot ID
CDN1A_MOUSE
Uniprot AC
P39689;
UniGene
Mm.195663;
GeneID
12575
KEGG
mmu:12575;
Mouse Cyclin-dependent kinase inhibitor 1 Protein SEQUENCE