Mouse Il6ra ELISA Kit

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  • Alternative name

    Mouse IL-6 receptor subunit alpha ELISA Kit;Mouse IL-6R subunit alpha ELISA Kit;Mouse IL-6R-alpha ELISA Kit;Mouse IL-6RA ELISA Kit;Mouse IL-6R 1 ELISA Kit;Mouse Il6r ELISA Kit;Mouse CD126 ELISA Kit;Mouse IL-6R ELISA Kit;Mouse interleukin 6 receptor, alpha ELISA Kit;Mouse interleukin-6 receptor subunit alpha ELISA Kit;Mouse IL-6 receptor alpha chain ELISA Kit;

  • Catalog
    E057483
  • species
    Mouse
  • GeneIl6ra
  • Standard CurveMouse Il6ra ELISA Kit
  • SpecificityThis assay recognizes recombinant and natural mouse IL-6 receptor subunit alpha. No significant cross-reactivity or interference was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of mouse IL-6 receptor subunit alpha is typically less than 33.0 pg/mL.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero.
  • Intended UseMouse Il6ra ELISA Kit allows for the in vitro quantitative determination of Il6ra , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-6 receptor subunit alpha. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-6 receptor subunit alpha and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IL-6 receptor subunit alpha, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IL-6 receptor subunit alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Interleukin-6 receptor subunit alpha Protein information
  • Uniprot ID IL6RA_MOUSE
  • Uniprot AC P22272;
  • UniGene Mm.2856;
  • GeneID 16194
  • KEGG mmu:16194;
  • Mouse Interleukin-6 receptor subunit alpha Protein SEQUENCE
  • SEQUENCE 460 AA; 50455 MW; F85C5906D08525C4 CRC64;

    MLTVGCTLLV ALLAAPAVAL VLGSCRALEV ANGTVTSLPG ATVTLICPGK

    EAAGNVTIHW VYSGSQNREW TTTGNTLVLR DVQLSDTGDY LCSLNDHLVG

    TVPLLVDVPP EEPKLSCFRK NPLVNAICEW RPSSTPSPTT KAVLFAKKIN

    TTNGKSDFQV PCQYSQQLKS FSCQVEILEG DKVYHIVSLC VANSVGSKSS

    HNEAFHSLKM VQPDPPANLV VSAIPGRPRW LKVSWQHPET WDPSYYLLQF

    QLRYRPVWSK EFTVLLLPVA QYQCVIHDAL RGVKHVVQVR GKEELDLGQW

    SEWSPEVTGT PWIAEPRTTP AGILWNPTQV SVEDSANHED QYESSTEATS

    VLAPVQESSS MSLPTFLVAG GSLAFGLLLC VFIILRLKQK WKSEAEKESK

    TTSPPPPPYS LGPLKPTFLL VPLLTPHSSG SDNTVNHSCL GVRDAQSPYD

    NSNRDYLFPR

  • UCSC uc008qaf.1; mouse.;



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