Human CSF2 ELISA Kit

Alternative name
Human GM-CSF ELISA Kit;Human Colony-stimulating factor ELISA Kit;Human CSF ELISA Kit;Human molgramostin ELISA Kit;Human sargramostim ELISA Kit;Human GMCSF ELISA Kit;Human colony stimulating factor 2 ELISA Kit;Human granulocyte-macrophage colony-stimulating factor ELISA Kit;Human colony stimulating factor 2 (granulocyte-macrophage) ELISA Kit;Human granulocyte macrophage-colony stimulating factor ELISA Kit;
Catalog
E017243
species
Human
GeneCSF2
SpecificityThe Human GM-CSF ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human GM-CSF proteins within the range of 31.3 pg/ml - 2000 pg/ml.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity7 pg/ml.
Intended UseHuman CSF2 ELISA Kit allows for the in vitro quantitative determination of CSF2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||The Human GM-CSF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human GM-CSF in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates, Urine. This assay employs an antibody specific for Human GM-CSF coated on a 96-well plate. Standards and samples are pipetted into the wells and GM-CSF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human GM-CSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GM-CSF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: Granulocyte macrophage colony stimulating factor (GM-CSF) is a pleiotropic cytokine with multiple effects on hematopoietic cells (1-4). It mobilizes CD34+ progenitor cells into the periphery and stimulates their proliferation, survival and differentiation into neutrophils,monocytes/ macrophages, eosinophils, and myeloid dendritic cells (4-8). On these terminallydifferentiated myeloid cells, GM-CSF is also needed for inducing their effector functions (7-10). In addition, GM-CSF has been shown to stimulate the proliferation and differentiation of the erythroid and megakaryoctye progenitor cells (4). GM-CSF is produced by a number of different cell types, including keratinocytes, mature and immature NK cells, type II alveolar cells), endothelial cells, monocytes, bone-marrow mesenchymal stem cells, CD4+ and CD8+ T cells, megakaryocytes, B cells, eosinophils, chondrocytes and fibroblasts. Human GM-CSF cDNA encodes a 144 amino acid (aa) residue precursor protein with a 17 aa putative signal peptide and a 127 aa mature protien (11 - 13). Natural GM-CSF is a monomer that contains both N- and O-linked glycosylation (14). Mature human GM-CSF shares approximately 55%, 63% and 68% aa sequence homology with mouse, rat and canine. GM-CSF, respectively. Human GM-CSF is not biologically active on mouse cells (11), but was reported to have some activity on canine cells (15). GM-CSF exerts its activity through binding to a high affinity receptor complex consisting of two membrane glycoproteins. The presence of the spliced variants in the heteromeric receptor complex can regulate the functions of the complex (16).
Human Granulocyte-macrophage colony-stimulating factor Protein information
Uniprot ID CSF2_HUMAN
Uniprot AC P04141; Q14CE8; Q2VPI8; Q8NFI6;
UniGene Hs.1349;
GeneID 1437
KEGG hsa:1437;
Human Granulocyte-macrophage colony-stimulating factor Protein SEQUENCE
SEQUENCE 144 AA; 16295 MW; 75D1E50506BCA7A8 CRC64;
MWLQSLLLLG TVACSISAPA RSPSPSTQPW EHVNAIQEAR RLLNLSRDTA
AEMNETVEVI SEMFDLQEPT CLQTRLELYK QGLRGSLTKL KGPLTMMASH
YKQHCPPTPE TSCATQIITF ESFKENLKDF LLVIPFDCWE PVQE
UCSC uc003kwf.5; human.;

Human CSF2 ELISA Kit

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