SpecificityThe assay recognizes natural human GM-CSF. To define specificity of this ELISA, several proteins were tested for cross reactivity. There was no cross reactivity observed for any protein tested (IL-1alpha, IL-1beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IFNgamma, TNFalpha).
SensitivityThe sensitivity, minimum detectable dose of GM-CSF using this GM-CSF ELISA kit was found to be <4.8pg/ml. This was determined by adding 2 standard deviations to the mean OD obtained when the zero standard was assayed 40 times.
Intended UseHuman GM-CSF ELISA Kit allows for the in vitro quantitative determination of GM-CSF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the assay: A capture Antibody highly specific for GM-CSF has been coated to the wells of the microtiter strip plate provided during manufacture. Binding of GM-CSF in samples and known standards to the capture antibodies and subsequent binding of the biotinylated anti- GMCSF secondary antibody to the analyte is completed during the same incubation period. Any excess unbound analyte and secondary antibody is removed. The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing. A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of GM-CSF present in the samples and standards. The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of GM-CSF in any sample tested.