Human AFP.L3 ELISA Kit

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  • Catalog
    E001701
  • species
    Human
  • GeneAFP.L3
  • Standard CurveHuman AFP.L3 ELISA Kit
  • Other Species Human AFP-L3 ELISA KitMouse AFP-L3 ELISA Kit
  • SpecificitySensitivity: The sensitivity in this assay is 0.1ng/ml Specificity: This assay has high sensitivity and excellent specificity for detection of AT2R1 AB. No significant cross-reactivity or interference between AT2R1 AB and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman AFP.L3 ELISA Kit allows for the in vitro quantitative determination of AFP.L3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyHuman ELISA Kit
  • Product Description
    specifical
    For Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma Intended Uses: This AT2R1 AB ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of AT2R1 AB in the sample, this AT2R1 AB ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus AT2R1 AB concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of AT2R1 AB utilizes a multiclonal anti-AT2R1 AB antibody and an AT2R1 AB-HRP conjugate. The assay sample and buffer are incubated together with AT2R1 AB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AT2R1 AB concentration since AT2R1 AB from samples and AT2R1 AB-HRP conjugate compete for the anti-AT2R1 AB antibody binding site. Since the number of sites is limited, as more sites are occupied by AT2R1 AB from the sample, fewer sites are left to bind AT2R1 AB-HRP conjugate. Standards of known AT2R1 AB concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of AT2R1 AB. The AT2R1 AB concentration in each sample is interpolated from this standard curve.


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