Human GC ELISA Kit

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  • Alternative name

    Human DBP ELISA Kit;Human VDB ELISA Kit;Human gc protein-derived macrophage activating factor ELISA Kit;Human Gc-MAF ELISA Kit;Human GcMAF ELISA Kit;Human gc-globulin ELISA Kit;Human Group-specific component ELISA Kit;Human Gc ELISA Kit;Human vitamin D-binding protein-macrophage activating factor ELISA Kit;Human DBP-maf ELISA Kit;Human DBP/GC ELISA Kit;Human GRD3 ELISA Kit;Human HEL-S-51 ELISA Kit;Human VDBG ELISA Kit;Human VDBP ELISA Kit;Human GC, vitamin D binding protein ELISA Kit;Human vitamin D-binding protein ELISA Kit;Human epididymis secretory protein Li 51 ELISA Kit;Human group-specific component (vitamin D binding protein) ELISA Kit;Human vitamin D-binding alpha-globulin ELISA Kit;

  • Catalog
    E016512
  • species
    Human
  • GeneGC
  • Standard CurveHuman GC ELISA Kit
  • Other Species Mouse DBP/GC ELISA KitMouse Gc ELISA KitBovine GC ELISA KitRabbit GC ELISA KitRat Gc ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman GC ELISA Kit allows for the in vitro quantitative determination of GC , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Principle of the Assay: GC ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GC. Standards or samples are then added to the microtiter plate wells and GC if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GC present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for GC are added to each well to "sandwich" the GC immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GC and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GC concentration in each sample is interpolated from this standard curve.
  • Human Vitamin D-binding protein Protein information
  • Uniprot ID VTDB_HUMAN
  • Uniprot AC P02774; B4DPP2; D6RAK8; Q16309; Q16310; Q53F31; Q6GTG1;
  • UniGene Hs.418497;
  • GeneID 2638
  • KEGG hsa:2638;
  • Human Vitamin D-binding protein Protein SEQUENCE
  • SEQUENCE 474 AA; 52964 MW; 6AD8F163B551F1E4 CRC64;

    MKRVLVLLLA VAFGHALERG RDYEKNKVCK EFSHLGKEDF TSLSLVLYSR

    KFPSGTFEQV SQLVKEVVSL TEACCAEGAD PDCYDTRTSA LSAKSCESNS

    PFPVHPGTAE CCTKEGLERK LCMAALKHQP QEFPTYVEPT NDEICEAFRK

    DPKEYANQFM WEYSTNYGQA PLSLLVSYTK SYLSMVGSCC TSASPTVCFL

    KERLQLKHLS LLTTLSNRVC SQYAAYGEKK SRLSNLIKLA QKVPTADLED

    VLPLAEDITN ILSKCCESAS EDCMAKELPE HTVKLCDNLS TKNSKFEDCC

    QEKTAMDVFV CTYFMPAAQL PELPDVELPT NKDVCDPGNT KVMDKYTFEL

    SRRTHLPEVF LSKVLEPTLK SLGECCDVED STTCFNAKGP LLKKELSSFI

    DKGQELCADY SENTFTEYKK KLAERLKAKL PDATPKELAK LVNKRSDFAS

    NCCSINSPPL YCDSEIDAEL KNIL

  • UCSC uc003hge.4; human. [P02774-1];




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