Human GC ELISA Kit

Alternative name
Human DBP ELISA Kit;Human VDB ELISA Kit;Human gc protein-derived macrophage activating factor ELISA Kit;Human Gc-MAF ELISA Kit;Human GcMAF ELISA Kit;Human gc-globulin ELISA Kit;Human Group-specific component ELISA Kit;Human Gc ELISA Kit;Human vitamin D-binding protein-macrophage activating factor ELISA Kit;Human DBP-maf ELISA Kit;Human DBP/GC ELISA Kit;Human GRD3 ELISA Kit;Human HEL-S-51 ELISA Kit;Human VDBG ELISA Kit;Human VDBP ELISA Kit;Human GC, vitamin D binding protein ELISA Kit;Human vitamin D-binding protein ELISA Kit;Human epididymis secretory protein Li 51 ELISA Kit;Human group-specific component (vitamin D binding protein) ELISA Kit;Human vitamin D-binding alpha-globulin ELISA Kit;
Catalog
E016512
species
Human
GeneGC
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman GC ELISA Kit allows for the in vitro quantitative determination of GC , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the Assay: GC ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GC. Standards or samples are then added to the microtiter plate wells and GC if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GC present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for GC are added to each well to "sandwich" the GC immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GC and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GC concentration in each sample is interpolated from this standard curve.
Human Vitamin D-binding protein Protein information
Uniprot ID VTDB_HUMAN
Uniprot AC P02774; B4DPP2; D6RAK8; Q16309; Q16310; Q53F31; Q6GTG1;
UniGene Hs.418497;
GeneID 2638
KEGG hsa:2638;
Human Vitamin D-binding protein Protein SEQUENCE
SEQUENCE 474 AA; 52964 MW; 6AD8F163B551F1E4 CRC64;
MKRVLVLLLA VAFGHALERG RDYEKNKVCK EFSHLGKEDF TSLSLVLYSR
KFPSGTFEQV SQLVKEVVSL TEACCAEGAD PDCYDTRTSA LSAKSCESNS
PFPVHPGTAE CCTKEGLERK LCMAALKHQP QEFPTYVEPT NDEICEAFRK
DPKEYANQFM WEYSTNYGQA PLSLLVSYTK SYLSMVGSCC TSASPTVCFL
KERLQLKHLS LLTTLSNRVC SQYAAYGEKK SRLSNLIKLA QKVPTADLED
VLPLAEDITN ILSKCCESAS EDCMAKELPE HTVKLCDNLS TKNSKFEDCC
QEKTAMDVFV CTYFMPAAQL PELPDVELPT NKDVCDPGNT KVMDKYTFEL
SRRTHLPEVF LSKVLEPTLK SLGECCDVED STTCFNAKGP LLKKELSSFI
DKGQELCADY SENTFTEYKK KLAERLKAKL PDATPKELAK LVNKRSDFAS
NCCSINSPPL YCDSEIDAEL KNIL
UCSC uc003hge.4; human. [P02774-1];

Category

Human GC ELISA Kit