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Alternative name
Flt1 protein ELISA KIT; Flt1 protein ELISA KIT; Soluble Fms-related tyrosine kinase 1/soluble vascular endothelial growth factor receptor 1 isoform sFlt1-11a ELISA KIT; Soluble fms-like tyrosine kinase 1flt1 ELISA KIT; sFlt1 ELISA KIT; sflt1 ELISA KIT
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Catalog
E035707
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species
Human
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GenesFLT1
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SpecificityThis assay has high sensitivity and excellent specificity for detection of sFLt-1. No significant cross-reactivity or interference between sFLt-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between sFLt-1 and all the analogues, therefore, cross reaction may still exist in some cases.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.1 ng/mL.
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Intended UseHuman sFLT1 ELISA Kit allows for the in vitro quantitative determination of sFLT1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: sFLt-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-sFLt-1 antibody and an sFLt-1-HRP conjugate. The assay sample and buffer are incubated together with sFLt-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sFLt-1 concentration since sFLt-1 from samples and sFLt-1-HRP conjugate compete for the anti-sFLt-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by sFLt-1 from the sample, fewer sites are left to bind sFLt-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sFLt-1 concentration in each sample is interpolated from this standard curve.
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