Human PIR ELISA Kit

Alternative name
Human probable quercetin 2,3-dioxygenase PIR ELISA Kit;Human probable quercetinase ELISA Kit;Human pirin ELISA Kit;Human pirin (iron-binding nuclear protein) ELISA Kit;
Catalog
E029425
species
Human
GenePIR
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman PIR ELISA Kit allows for the in vitro quantitative determination of PIR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalPrinciple of the Assay: PIR ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PIR. Standards or samples are then added to the microtiter plate wells and PIR if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PIR present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for PIR are added to each well to "sandwich" the PIR immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PIR and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PIR concentration in each sample is interpolated from this standard curve
Human Pirin Protein information
Uniprot ID PIR_HUMAN
Uniprot AC O00625; Q5U0G0; Q6FHD2;
UniGene Hs.495728; Hs.732521;
GeneID 8544
KEGG hsa:8544;
Human Pirin Protein SEQUENCE
SEQUENCE 290 AA; 32113 MW; D06AC100F356496D CRC64;
MGSSKKVTLS VLSREQSEGV GARVRRSIGR PELKNLDPFL LFDEFKGGRP
GGFPDHPHRG FETVSYLLEG GSMAHEDFCG HTGKMNPGDL QWMTAGRGIL
HAEMPCSEEP AHGLQLWVNL RSSEKMVEPQ YQELKSEEIP KPSKDGVTVA
VISGEALGIK SKVYTRTPTL YLDFKLDPGA KHSQPIPKGW TSFIYTISGD
VYIGPDDAQQ KIEPHHTAVL GEGDSVQVEN KDPKRSHFVL IAGEPLREPV
IQHGPFVMNT NEEISQAILD FRNAKNGFER AKTWKSKIGN
UCSC uc004cwu.5; human.;

Category

Human PIR ELISA Kit

Human probable quercetin 2,3-dioxygenase PIR ELISA Kit;Human probable quercetinase ELISA Kit;Human pirin ELISA Kit;Human pirin (iron-binding nuclear protein) ELISA Kit;