Human PIR ELISA Kit

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  • Alternative name

    Human probable quercetin 2,3-dioxygenase PIR ELISA Kit;Human probable quercetinase ELISA Kit;Human pirin ELISA Kit;Human pirin (iron-binding nuclear protein) ELISA Kit;

  • Catalog
    E029425
  • species
    Human
  • GenePIR
  • Standard CurveHuman PIR ELISA Kit
  • Other Species Mouse Pir ELISA KitRat Pir ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman PIR ELISA Kit allows for the in vitro quantitative determination of PIR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical
    Principle of the Assay: PIR ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PIR. Standards or samples are then added to the microtiter plate wells and PIR if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PIR present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for PIR are added to each well to "sandwich" the PIR immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PIR and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PIR concentration in each sample is interpolated from this standard curve
  • Human Pirin Protein information
  • Uniprot ID PIR_HUMAN
  • Uniprot AC O00625; Q5U0G0; Q6FHD2;
  • UniGene Hs.495728; Hs.732521;
  • GeneID 8544
  • KEGG hsa:8544;
  • Human Pirin Protein SEQUENCE
  • SEQUENCE 290 AA; 32113 MW; D06AC100F356496D CRC64;

    MGSSKKVTLS VLSREQSEGV GARVRRSIGR PELKNLDPFL LFDEFKGGRP

    GGFPDHPHRG FETVSYLLEG GSMAHEDFCG HTGKMNPGDL QWMTAGRGIL

    HAEMPCSEEP AHGLQLWVNL RSSEKMVEPQ YQELKSEEIP KPSKDGVTVA

    VISGEALGIK SKVYTRTPTL YLDFKLDPGA KHSQPIPKGW TSFIYTISGD

    VYIGPDDAQQ KIEPHHTAVL GEGDSVQVEN KDPKRSHFVL IAGEPLREPV

    IQHGPFVMNT NEEISQAILD FRNAKNGFER AKTWKSKIGN

  • UCSC uc004cwu.5; human.;


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