Human EIF6 ELISA Kit

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  • Alternative name

    Eukaryotic translation initiation factor 6 ELISA KIT; B4 integrin interactor ELISA KIT; CAB ELISA KIT; p27(BBP)Eif6 ELISA KIT; Itgb4bp ELISA KIT; eIF-6 ELISA KIT

  • Catalog
    E013786
  • species
    Human
  • GeneEIF6
  • Standard CurveHuman EIF6 ELISA Kit
  • Other Species Mouse EIF6 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Eukaryotic Translation Initiation Factor 6 (EIF6). No significant cross-reactivity or interference between Eukaryotic Translation Initiation Factor 6 (EIF6) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.123ng/mL.
  • Intended UseHuman EIF6 ELISA Kit allows for the in vitro quantitative determination of EIF6 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Eukaryotic Translation Initiation Factor 6 (EIF6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Eukaryotic Translation Initiation Factor 6 (EIF6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Eukaryotic Translation Initiation Factor 6 (EIF6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Eukaryotic Translation Initiation Factor 6 (EIF6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.


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