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  • Alternative name

    Carcinoembryonic antigen-related cell adhesion molecule 5 ELISA KIT; Carcinoembryonic antigen ELISA KIT; CEA ELISA KIT; Meconium antigen 100 ELISA KIT; CD_antigen: CD66eCEACAM5 ELISA KIT; CEA ELISA KIT; CEA ELISA KIT

  • Catalog
  • species
  • GeneCEA
  • Standard CurveHuman CEA ELISA Kit
  • Other Species Mouse CEA ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Carcinoembryonic Antigen (CEA). No significant cross-reactivity or interference between Carcinoembryonic Antigen (CEA) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 7.1pg/mL.
  • Intended UseHuman CEA ELISA Kit allows for the in vitro quantitative determination of CEA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carcinoembryonic Antigen (CEA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carcinoembryonic Antigen (CEA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carcinoembryonic Antigen (CEA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carcinoembryonic Antigen (CEA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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