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Human SHC1 ELISA Kit

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  • Alternative name

    Human SHC-transforming protein 3 ELISA Kit;Human SHC-transforming protein A ELISA Kit;Human Src homology 2 domain-containing-transforming protein C1 ELISA Kit;Human SH2 domain protein C1 ELISA Kit;Human SHC ELISA Kit;Human SHCA ELISA Kit;Human SHC adaptor protein 1 ELISA Kit;Human SHC-transforming protein 1 ELISA Kit;Human SHC (Src homology 2 domain containing) transforming protein 1 ELISA Kit;
  • Catalog
    E035115
  • species
    Human
  • GeneSHC1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of SHC-Transforming Protein 1 (SHC1). No significant cross-reactivity or interference between SHC-Transforming Protein 1 (SHC1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.117ng/mL.
  • Intended UseHuman SHC1 ELISA Kit allows for the in vitro quantitative determination of SHC1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SHC-Transforming Protein 1 (SHC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to SHC-Transforming Protein 1 (SHC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SHC-Transforming Protein 1 (SHC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SHC-Transforming Protein 1 (SHC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human SHC-transforming protein 1 Protein information
  • Uniprot ID SHC1_HUMAN
  • Uniprot AC Q5T186; Q8N4K5; Q96CL1;
  • UniGene Hs.433795;
  • GeneID 6464
  • KEGG hsa:6464;
  • Human SHC-transforming protein 1 Protein SEQUENCE

  • SEQUENCE 583 AA; 62822 MW; 7EFA5CB185A548D1 CRC64;

    MDLLPPKPKY NPLRNESLSS LEEGASGSTP PEELPSPSAS SLGPILPPLP

    GDDSPTTLCS FFPRMSNLRL ANPAGGRPGS KGEPGRAADD GEGIVGAAMP

    DSGPLPLLQD MNKLSGGGGR RTRVEGGQLG GEEWTRHGSF VNKPTRGWLH

    PNDKVMGPGV SYLVRYMGCV EVLQSMRALD FNTRTQVTRE AISLVCEAVP

    GAKGATRRRK PCSRPLSSIL GRSNLKFAGM PITLTVSTSS LNLMAADCKQ

    IIANHHMQSI SFASGGDPDT AEYVAYVAKD PVNQRACHIL ECPEGLAQDV

    ISTIGQAFEL RFKQYLRNPP KLVTPHDRMA GFDGSAWDEE EEEPPDHQYY

    NDFPGKEPPL GGVVDMRLRE GAAPGAARPT APNAQTPSHL GATLPVGQPV

    GGDPEVRKQM PPPPPCPGRE LFDDPSYVNV QNLDKARQAV GGAGPPNPAI

    NGSAPRDLFD MKPFEDALRV PPPPQSVSMA EQLRGEPWFH GKLSRREAEA

    LLQLNGDFLV RESTTTPGQY VLTGLQSGQP KHLLLVDPEG VVRTKDHRFE

    SVSHLISYHM DNHLPIISAG SELCLQQPVE RKL

  • UCSC uc001ffv.4; human. [P29353-1];



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