Human WNT5A ELISA Kit

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  • Alternative name

    Human hWNT5A ELISA Kit;Human Wnt family member 5A ELISA Kit;Human protein Wnt-5a ELISA Kit;Human WNT-5A protein ELISA Kit;Human wingless-type MMTV integration site family, member 5A ELISA Kit;

  • Catalog
    E031926
  • species
    Human
  • GeneWNT5A
  • Other Species Mouse Wnt5a ELISA KitTurkey WNT5A ELISA KitRabbit WNT5A ELISA KitRat Wnt5a ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.094ng/ml
  • Intended UseHuman WNT5A ELISA Kit allows for the in vitro quantitative determination of WNT5A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human Protein Wnt-5a antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human Protein Wnt-5a antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Protein Wnt-5a amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human Protein Wnt-5a can be calculated.
  • Human Protein Wnt-5a Protein information
  • Uniprot ID WNT5A_HUMAN
  • Uniprot AC P41221; A8K4A4; Q6P278;
  • UniGene Hs.643085;
  • GeneID 7474
  • KEGG hsa:7474;
  • Human Protein Wnt-5a Protein SEQUENCE
  • SEQUENCE 380 AA; 42339 MW; 50E73AC7FE96C7B5 CRC64;

    MKKSIGILSP GVALGMAGSA MSSKFFLVAL AIFFSFAQVV IEANSWWSLG

    MNNPVQMSEV YIIGAQPLCS QLAGLSQGQK KLCHLYQDHM QYIGEGAKTG

    IKECQYQFRH RRWNCSTVDN TSVFGRVMQI GSRETAFTYA VSAAGVVNAM

    SRACREGELS TCGCSRAARP KDLPRDWLWG GCGDNIDYGY RFAKEFVDAR

    ERERIHAKGS YESARILMNL HNNEAGRRTV YNLADVACKC HGVSGSCSLK

    TCWLQLADFR KVGDALKEKY DSAAAMRLNS RGKLVQVNSR FNSPTTQDLV

    YIDPSPDYCV RNESTGSLGT QGRLCNKTSE GMDGCELMCC GRGYDQFKTV

    QTERCHCKFH WCCYVKCKKC TEIVDQFVCK

  • UCSC uc003dhn.5; human. [P41221-1];



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