Human E2F1 ELISA Kit

Alternative name
Human E2F-1 ELISA Kit;Human PBR3 ELISA Kit;Human retinoblastoma-associated protein 1 ELISA Kit;Human RBAP-1 ELISA Kit;Human retinoblastoma-binding protein 3 ELISA Kit;Human RBBP-3 ELISA Kit;Human PRB-binding protein E2F-1 ELISA Kit;Human RBBP3 ELISA Kit;Human RBAP1 ELISA Kit;Human RBP3 ELISA Kit;Human E2F transcription factor 1 ELISA Kit;Human transcription factor E2F1 ELISA Kit;
Catalog
E012623
species
Human
GeneE2F1
Standard Curve
Other Species Mouse E2f1 ELISA Kit Chicken E2F1 ELISA Kit Rat E2f1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of E2F1. No significant cross-reactivity or interference between E2F1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human E2F1 is typically less than 0.054g/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by addin
Intended UseHuman E2F1 ELISA Kit allows for the in vitro quantitative determination of E2F1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to E2F1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to E2F1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain E2F1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of E2F1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Transcription factor E2F1 Protein information
Uniprot ID E2F1_HUMAN
Uniprot AC Q01094; Q13143; Q92768;
UniGene Hs.654393;
GeneID 1869
KEGG hsa:1869;
Human Transcription factor E2F1 Protein SEQUENCE
SEQUENCE 437 AA; 46920 MW; 003B3F654F0C60DF CRC64;
MALAGAPAGG PCAPALEALL GAGALRLLDS SQIVIISAAQ DASAPPAPTG
PAAPAAGPCD PDLLLFATPQ APRPTPSAPR PALGRPPVKR RLDLETDHQY
LAESSGPARG RGRHPGKGVK SPGEKSRYET SLNLTTKRFL ELLSHSADGV
VDLNWAAEVL KVQKRRIYDI TNVLEGIQLI AKKSKNHIQW LGSHTTVGVG
GRLEGLTQDL RQLQESEQQL DHLMNICTTQ LRLLSEDTDS QRLAYVTCQD
LRSIADPAEQ MVMVIKAPPE TQLQAVDSSE NFQISLKSKQ GPIDVFLCPE
ETVGGISPGK TPSQEVTSEE ENRATDSATI VSPPPSSPPS SLTTDPSQSL
LSLEQEPLLS RMGSLRAPVD EDRLSPLVAA DSLLEHVRED FSGLLPEEFI
SLSPPHEALD YHFGLEEGEG IRDLFDCDFG DLTPLDF
UCSC uc002wzu.5; human.;

Related products with Human E2F1 ELISA Kit

anti-E2F1 antibody

Category

Human E2F1 ELISA Kit