Intended UseHuman TNFRSF14/HVEM ELISA Kit allows for the in vitro quantitative determination of TNFRSF14/HVEM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HVEM. During the reaction,HVEM in the sample or standard competes with a fixed amount of biotin-labeled HVEM for sites on a pre-coated Monoclonal antibody specific to HVEM. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of HVEM in the samples is then determined by comparing the O.D. of the samples to the standard curve.