Intended UseHuman TNFRSF14/HVEM ELISA Kit allows for the in vitro quantitative determination of TNFRSF14/HVEM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HVEM. During the reaction,HVEM in the sample or standard competes with a fixed amount of biotin-labeled HVEM for sites on a pre-coated Monoclonal antibody specific to HVEM. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of HVEM in the samples is then determined by comparing the O.D. of the samples to the standard curve.
No manual or datasheet available for this product.