Human RAC1 ELISA Kit

Alternative name
Human cell migration-inducing gene 5 protein ELISA Kit;Human ras-like protein TC25 ELISA Kit;Human p21-Rac1 ELISA Kit;Human TC25 ELISA Kit;Human MIG5 ELISA Kit;Human Rac-1 ELISA Kit;Human TC-25 ELISA Kit;Human ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) ELISA Kit;Human ras-related C3 botulinum toxin substrate 1 ELISA Kit;
Catalog
E032889
species
Human
GeneRAC1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Ras Related C3 Botulinum Toxin Substrate 1 (Rac1). No significant cross-reactivity or interference between Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.056ng/mL.
Intended UseHuman RAC1 ELISA Kit allows for the in vitro quantitative determination of RAC1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ras Related C3 Botulinum Toxin Substrate 1 (Rac1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ras Related C3 Botulinum Toxin Substrate 1 (Rac1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ras Related C3 Botulinum Toxin Substrate 1 (Rac1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Ras-related C3 botulinum toxin substrate 1 Protein information
Uniprot ID RAC1_HUMAN
Uniprot AC P63000; O95501; P15154; Q3Y4D3; Q5JAA8; Q9BTB4;
UniGene Hs.413812;
GeneID 5879
KEGG hsa:5879;
Human Ras-related C3 botulinum toxin substrate 1 Protein SEQUENCE
SEQUENCE 192 AA; 21450 MW; ACEDF83A45E5EA67 CRC64;
MQAIKCVVVG DGAVGKTCLL ISYTTNAFPG EYIPTVFDNY SANVMVDGKP
VNLGLWDTAG QEDYDRLRPL SYPQTDVFLI CFSLVSPASF ENVRAKWYPE
VRHHCPNTPI ILVGTKLDLR DDKDTIEKLK EKKLTPITYP QGLAMAKEIG
AVKYLECSAL TQRGLKTVFD EAIRAVLCPP PVKKRKRKCL LL
UCSC uc003spw.4; human.;

Human RAC1 ELISA Kit

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