Human GFAP ELISA Kit

Alternative name
Human GFAP ELISA Kit;Human ALXDRD ELISA Kit;Human glial fibrillary acidic protein ELISA Kit;
Catalog
E016476
species
Human
GeneGFAP
Other Species Mouse Gfap ELISA Kit Bovine GFAP ELISA Kit Rat Gfap ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of human GFAP. No significant cross-reactivity or interference between human GFAP and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human GFAP is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
Intended UseHuman GFAP ELISA Kit allows for the in vitro quantitative determination of GFAP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GFAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GFAP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GFAP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GFAP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Glial fibrillary acidic protein Protein information
Uniprot ID GFAP_HUMAN
Uniprot AC Q7Z5J7; Q96KS4; Q96P18; Q9UFD0;
UniGene Hs.514227;
GeneID 2670
KEGG hsa:2670;
Human Glial fibrillary acidic protein Protein SEQUENCE
SEQUENCE 432 AA; 49880 MW; E6C3B3454C3F1250 CRC64;
MERRRITSAA RRSYVSSGEM MVGGLAPGRR LGPGTRLSLA RMPPPLPTRV
DFSLAGALNA GFKETRASER AEMMELNDRF ASYIEKVRFL EQQNKALAAE
LNQLRAKEPT KLADVYQAEL RELRLRLDQL TANSARLEVE RDNLAQDLAT
VRQKLQDETN LRLEAENNLA AYRQEADEAT LARLDLERKI ESLEEEIRFL
RKIHEEEVRE LQEQLARQQV HVELDVAKPD LTAALKEIRT QYEAMASSNM
HEAEEWYRSK FADLTDAAAR NAELLRQAKH EANDYRRQLQ SLTCDLESLR
GTNESLERQM REQEERHVRE AASYQEALAR LEEEGQSLKD EMARHLQEYQ
DLLNVKLALD IEIATYRKLL EGEENRITIP VQTFSNLQIR ETSLDTKSVS
EGHLKRNIVV KTVEMRDGEV IKESKQEHKD VM
UCSC uc002ihq.3; human. [P14136-1];

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Category

Human GFAP ELISA Kit

Human GFAP ELISA Kit;Human ALXDRD ELISA Kit;Human glial fibrillary acidic protein ELISA Kit;