Human NBL1 ELISA Kit

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  • Alternative name

    Human DAN domain family member 1 ELISA Kit;Human Protein N03 ELISA Kit;Human Zinc finger protein DAN ELISA Kit;Human DAN ELISA Kit;Human DAND1 ELISA Kit;Human D1S1733E ELISA Kit;Human NB ELISA Kit;Human NO3 ELISA Kit;Human neuroblastoma 1, DAN family BMP antagonist ELISA Kit;Human neuroblastoma suppressor of tumorigenicity 1 ELISA Kit;Human differential screening-selected gene aberrant in neuroblastoma ELISA Kit;Human neuroblastoma candidate region, suppression of tumorigenicity 1 ELISA Kit;

  • Catalog
    E011372
  • species
    Human
  • GeneNBL1
  • Standard CurveHuman NBL1 ELISA Kit
  • Other Species Mouse Nbl1 ELISA KitChicken NBL1 ELISA KitRat Nbl1 ELISA Kit
  • SpecificityNatural and recombinant human DAN
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity Sample For quantitative detection of human DAN in cell culture supernates, serum and plasma
  • Intended UseHuman NBL1 ELISA Kit allows for the in vitro quantitative determination of NBL1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: Human DAN ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for DAN has been precoated onto 96-well plates. Standards (NSO, A17-D181) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DAN is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human DAN amount of sample captured in plate. Background: Differential screening-selected gene aberrative in neuroblastoma (DAN) is a member of the DAN family of secreted glycoproteins that are putative BMP antagonists. The NBL1 gene, also known as DAN, is originally cloned from a normal rat fibroblast cDNA library by a differential screening method. The human DAN gene is mapped to chromosome 1p36.13-p36. It is found that the DAN gene possesses a tumor suppressive activity when overexpressed in v-src transformed cells.
  • Human Neuroblastoma suppressor of tumorigenicity 1 Protein information
  • Uniprot ID NBL1_HUMAN
  • Uniprot AC P41271; A3KFI7; Q5TGZ2; Q5U0N4; Q96L68;
  • UniGene Hs.466662; Hs.654502;
  • GeneID 4681
  • KEGG hsa:4681;
  • Human Neuroblastoma suppressor of tumorigenicity 1 Protein SEQUENCE
  • SEQUENCE 181 AA; 19408 MW; AE9E3264EF38DAD7 CRC64;

    MMLRVLVGAV LPAMLLAAPP PINKLALFPD KSAWCEAKNI TQIVGHSGCE

    AKSIQNRACL GQCFSYSVPN TFPQSTESLV HCDSCMPAQS MWEIVTLECP

    GHEEVPRVDK LVEKILHCSC QACGKEPSHE GLSVYVQGED GPGSQPGTHP

    HPHPHPHPGG QTPEPEDPPG APHTEEEGAE D

  • UCSC uc001bcj.3; human. [P41271-1];


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