Human CST1 ELISA Kit

Alternative name
Human cystain-SA-I ELISA Kit;Human Cystatin-1 ELISA Kit;Human salivary cystatin-SA-1 ELISA Kit;Human cystatin SN ELISA Kit;Human cystatin-SN ELISA Kit;Human cystatin 1 ELISA Kit;Human cystatin SA-I ELISA Kit;Human cysteine proteinase inhibitor, type 2 family ELISA Kit;
Catalog
E010778
species
Human
GeneCST1
Standard Curve
Other Species Mouse CST1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of CST1. No significant cross-reactivity or interference between CST1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CST1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CST1 ELISA Kit allows for the in vitro quantitative determination of CST1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalPrinciple of the assay: CST1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CST1 antibody and an CST1-HRP conjugate. The assay sample and buffer are incubated together with CST1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CST1 concentration since CST1 from samples and CST1-HRP conjugate compete for the anti-CST1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CST1 from the sample, fewer sites are left to bind CST1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CST1 concentration in each sample is interpolated from this standard curve.
Human Cystatin-SN Protein information
Uniprot ID CYTN_HUMAN
Uniprot AC P01037; Q96LE6; Q9UCQ6;
UniGene Hs.123114;
GeneID 1469
KEGG hsa:1469;
Human Cystatin-SN Protein SEQUENCE
SEQUENCE 141 AA; 16388 MW; 9F9934704D6C077D CRC64;
MAQYLSTLLL LLATLAVALA WSPKEEDRII PGGIYNADLN DEWVQRALHF
AISEYNKATK DDYYRRPLRV LRARQQTVGG VNYFFDVEVG RTICTKSQPN
LDTCAFHEQP ELQKKQLCSF EIYEVPWENR RSLVKSRCQE S
UCSC uc002wtp.3; human.;

Related products with Human CST1 ELISA Kit

anti-CST1 antibody

Category

Human CST1 ELISA Kit

Human cystain-SA-I ELISA Kit;Human Cystatin-1 ELISA Kit;Human salivary cystatin-SA-1 ELISA Kit;Human cystatin SN ELISA Kit;Human cystatin-SN ELISA Kit;Human cystatin 1 ELISA Kit;Human cystatin SA-I ELISA Kit;Human cysteine proteinase inhibitor, type 2 family ELISA Kit;