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Alternative name
CD3gamma/delta ELISA KIT; CD3gamma/delta ELISA KIT; Cd3 protein ELISA KIT; T-cell surface glycoprotein CD3 gamma/delta chain homologcd3g ELISA KIT; cd3 ELISA KIT; cd3g-a ELISA KIT
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Catalog
E009690
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species
Human
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GeneCD3
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Standard Curve
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Other Species
Mouse CD3 ELISA Kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of human CD3. No significant cross-reactivity or interference between human CD3 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of human CD3 is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
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Intended UseHuman CD3 ELISA Kit allows for the in vitro quantitative determination of CD3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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