Human FAH ELISA Kit

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  • Alternative name

    Human FAA ELISA Kit;Human beta-diketonase ELISA Kit;Human fumarylacetoacetate hydrolase ELISA Kit;Human fumarylacetoacetase ELISA Kit;Human fumarylacetoacetate hydrolase (fumarylacetoacetase) ELISA Kit;

  • Catalog
    E015680
  • species
    Human
  • GeneFAH
  • Standard CurveHuman FAH ELISA Kit
  • Other Species Mouse Fah ELISA KitBovine FAH ELISA KitRat Fah ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Fumarylacetoacetate Hydrolase (FAH). No significant cross-reactivity or interference between Fumarylacetoacetate Hydrolase (FAH) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.058ng/mL.
  • Intended UseHuman FAH ELISA Kit allows for the in vitro quantitative determination of FAH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fumarylacetoacetate Hydrolase (FAH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fumarylacetoacetate Hydrolase (FAH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fumarylacetoacetate Hydrolase (FAH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fumarylacetoacetate Hydrolase (FAH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Fumarylacetoacetase Protein information
  • Uniprot ID FAAA_HUMAN
  • Uniprot AC P16930; B2R9X1; D3DW95; Q53XA7;
  • UniGene Hs.73875;
  • GeneID 2184
  • KEGG hsa:2184;
  • Human Fumarylacetoacetase Protein SEQUENCE
  • SEQUENCE 419 AA; 46374 MW; 12EA8D8074C55BB2 CRC64;

    MSFIPVAEDS DFPIHNLPYG VFSTRGDPRP RIGVAIGDQI LDLSIIKHLF

    TGPVLSKHQD VFNQPTLNSF MGLGQAAWKE ARVFLQNLLS VSQARLRDDT

    ELRKCAFISQ ASATMHLPAT IGDYTDFYSS RQHATNVGIM FRDKENALMP

    NWLHLPVGYH GRASSVVVSG TPIRRPMGQM KPDDSKPPVY GACKLLDMEL

    EMAFFVGPGN RLGEPIPISK AHEHIFGMVL MNDWSARDIQ KWEYVPLGPF

    LGKSFGTTVS PWVVPMDALM PFAVPNPKQD PRPLPYLCHD EPYTFDINLS

    VNLKGEGMSQ AATICKSNFK YMYWTMLQQL THHSVNGCNL RPGDLLASGT

    ISGPEPENFG SMLELSWKGT KPIDLGNGQT RKFLLDGDEV IITGYCQGDG

    YRIGFGQCAG KVLPALLPS

  • UCSC uc002bfm.3; human. [P16930-1];


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