Human CERK ELISA Kit

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  • Alternative name

    Human hCERK ELISA Kit;Human acylsphingosine kinase ELISA Kit;Human lipid kinase 4 ELISA Kit;Human LK4 ELISA Kit;Human KIAA1646 ELISA Kit;Human dA59H18.2 ELISA Kit;Human dA59H18.3 ELISA Kit;Human ceramide kinase ELISA Kit;Human lipid kinase LK4 ELISA Kit;

  • Catalog
    E007837
  • species
    Human
  • GeneCERK
  • Standard CurveHuman CERK ELISA Kit
  • Other Species Mouse Cerk ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CERK. No significant cross-reactivity or interference between CERK and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CERK and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman CERK ELISA Kit allows for the in vitro quantitative determination of CERK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical
    Principle of the assay: CERK ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CERK antibody and an CERK-HRP conjugate. The assay sample and buffer are incubated together with CERK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CERK concentration since CERK from samples and CERK-HRP conjugate compete for the anti-CERK antibody binding site. Since the number of sites is limited, as more sites are occupied by CERK from the sample, fewer sites are left to bind CERK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CERK concentration in each sample is interpolated from this standard curve.
  • Human Ceramide kinase Protein information
  • Uniprot ID CERK1_HUMAN
  • Uniprot AC Q8TCT0; A0JNT4; A8K611; Q6NX59; Q9BYB3; Q9UGE5;
  • UniGene Hs.200668;
  • GeneID 64781
  • KEGG hsa:64781;
  • Human Ceramide kinase Protein SEQUENCE
  • SEQUENCE 537 AA; 59977 MW; 3DBFC0ED8D679F7F CRC64;

    MGATGAAEPL QSVLWVKQQR CAVSLEPARA LLRWWRSPGP GAGAPGADAC

    SVPVSEIIAV EETDVHGKHQ GSGKWQKMEK PYAFTVHCVK RARRHRWKWA

    QVTFWCPEEQ LCHLWLQTLR EMLEKLTSRP KHLLVFINPF GGKGQGKRIY

    ERKVAPLFTL ASITTDIIVT EHANQAKETL YEINIDKYDG IVCVGGDGMF

    SEVLHGLIGR TQRSAGVDQN HPRAVLVPSS LRIGIIPAGS TDCVCYSTVG

    TSDAETSALH IVVGDSLAMD VSSVHHNSTL LRYSVSLLGY GFYGDIIKDS

    EKKRWLGLAR YDFSGLKTFL SHHCYEGTVS FLPAQHTVGS PRDRKPCRAG

    CFVCRQSKQQ LEEEQKKALY GLEAAEDVEE WQVVCGKFLA INATNMSCAC

    RRSPRGLSPA AHLGDGSSDL ILIRKCSRFN FLRFLIRHTN QQDQFDFTFV

    EVYRVKKFQF TSKHMEDEDS DLKEGGKKRF GHICSSHPSC CCTVSNSSWN

    CDGEVLHSPA IEVRVHCQLV RLFARGIEEN PKPDSHS

  • UCSC uc003bia.4; human. [Q8TCT0-1];




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