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Human MGMT ELISA Kit

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  • Alternative name

    Human 6-O-methylguanine-DNA methyltransferase ELISA Kit;Human MGMT ELISA Kit;Human O-6-methylguanine-DNA-alkyltransferase ELISA Kit;Human O-6-methylguanine-DNA methyltransferase ELISA Kit;Human methylated-DNA--protein-cysteine methyltransferase ELISA Kit;Human O6-methylguanine-DNA methyltransferase ELISA Kit;Human methylguanine-DNA methyltransferase ELISA Kit;
  • Catalog
    E027191
  • species
    Human
  • GeneMGMT
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of O-6-Methylguanine DNA Methyltransferase (MGMT). No significant cross-reactivity or interference between O-6-Methylguanine DNA Methyltransferase (MGMT) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.060ng/mL.
  • Intended UseHuman MGMT ELISA Kit allows for the in vitro quantitative determination of MGMT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to O-6-Methylguanine DNA Methyltransferase (MGMT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to O-6-Methylguanine DNA Methyltransferase (MGMT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain O-6-Methylguanine DNA Methyltransferase (MGMT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of O-6-Methylguanine DNA Methyltransferase (MGMT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Methylated-DNA--protein-cysteine methyltransferase Protein information
  • Uniprot ID MGMT_HUMAN
  • Uniprot AC P16455; Q5VY78;
  • UniGene Hs.501522;
  • GeneID 4255
  • Human Methylated-DNA--protein-cysteine methyltransferase Protein SEQUENCE

  • SEQUENCE 207 AA; 21646 MW; 76BBF19DFC4512D6 CRC64;

    MDKDCEMKRT TLDSPLGKLE LSGCEQGLHE IKLLGKGTSA ADAVEVPAPA

    AVLGGPEPLM QCTAWLNAYF HQPEAIEEFP VPALHHPVFQ QESFTRQVLW

    KLLKVVKFGE VISYQQLAAL AGNPKAARAV GGAMRGNPVP ILIPCHRVVC

    SSGAVGNYSG GLAVKEWLLA HEGHRLGKPG LGGSSGLAGA WLKGAGATSG

    SPPAGRN

  • UCSC uc001lkh.3; human.;



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