Human CD27BP ELISA Kit

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  • Alternative name

    CD27 antigen ELISA KIT; CD27L receptor ELISA KIT; T-cell activation antigen CD27 ELISA KIT; T14 ELISA KIT; Tumor necrosis factor receptor superfamily member 7 ELISA KIT; CD_antigen: CD27CD27 ELISA KIT; TNFRSF7 ELISA KIT

  • Catalog
  • species
  • GeneCD27BP
  • Standard CurveHuman CD27BP ELISA Kit
  • Other Species Mouse CD27BP ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CD27 Binding Protein (CD27BP). No significant cross-reactivity or interference between CD27 Binding Protein (CD27BP) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.068ng/mL.
  • Intended UseHuman CD27BP ELISA Kit allows for the in vitro quantitative determination of CD27BP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CD27 Binding Protein (CD27BP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CD27 Binding Protein (CD27BP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CD27 Binding Protein (CD27BP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CD27 Binding Protein (CD27BP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.


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