Human C5b ELISA Kit

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  • Alternative name

    Complement C5 ELISA KIT; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4Cleaved into the following 4 chains:Complement C5 beta chain ELISA KIT; Complement C5 alpha chain ELISA KIT; C5a anaphylatoxin ELISA KIT; Complement C5 alpha' chainC5 ELISA KIT; CPAMD4 ELISA KIT

  • Catalog
  • species
  • GeneC5b
  • Other Species Human C5B 9 ELISA KitHuman C5B-8 ELISA KitHuman TCC C5b 9 ELISA KitHuman TCC C5b-9 ELISA KitHuman C5b-9 ELISA KitMouse C5B ELISA KitMouse C5B-9 ELISA KitMouse C5B-8 ELISA KitMouse TCC C5b-9 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human C5b. No significant cross-reactivity or interference between Human C5b and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human C5b and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.469ng/mL
  • Intended UseHuman C5b ELISA Kit allows for the in vitro quantitative determination of C5b , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of C5b in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C5b. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human C5b and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C5b, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human C5b. You can calculate the concentration of Human C5b in the samples by comparing the OD of the samples to the standard curve.

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