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Human PI4KA ELISA Kit

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  • Alternative name

    Human PI4-kinase alpha ELISA Kit;Human PI4K-ALPHA ELISA Kit;Human ptdIns-4-kinase alpha ELISA Kit;Human PIK4 ELISA Kit;Human PIK4CA ELISA Kit;Human PMGYCHA ELISA Kit;Human pi4K230 ELISA Kit;Human phosphatidylinositol 4-kinase alpha ELISA Kit;Human phosphatidylinositol 4-kinase 230 ELISA Kit;Human phosphatidylinositol 4-kinase, catalytic, alpha ELISA Kit;Human phosphatidylinositol 4-kinase, type III, alpha ELISA Kit;Human testicular secretory protein Li 35 ELISA Kit;
  • Catalog
    E028971
  • species
    Human
  • GenePI4KA
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Phosphatidylinositol-4-Kinase Catalytic Alpha (PI4Ka). No significant cross-reactivity or interference between Phosphatidylinositol-4-Kinase Catalytic Alpha (PI4Ka) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.27ng/mL.
  • Intended UseHuman PI4KA ELISA Kit allows for the in vitro quantitative determination of PI4KA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphatidylinositol-4-Kinase Catalytic Alpha (PI4Ka). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphatidylinositol-4-Kinase Catalytic Alpha (PI4Ka). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphatidylinositol-4-Kinase Catalytic Alpha (PI4Ka), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphatidylinositol-4-Kinase Catalytic Alpha (PI4Ka) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Phosphatidylinositol 4-kinase alpha Protein information
  • Uniprot ID PI4KA_HUMAN
  • Uniprot AC P42356; Q7Z625; Q9UPG2;
  • UniGene Hs.529438; Hs.731386;
  • GeneID 5297
  • KEGG hsa:5297;
  • Human Phosphatidylinositol 4-kinase alpha Protein SEQUENCE

  • SEQUENCE 2102 AA; 236830 MW; ED4C3C9EA79E6B5D CRC64;

    MAAAPARGGG GGGGGGGGCS GSGSSASRGF YFNTVLSLAR SLAVQRPASL

    EKVQKLLCMC PVDFHGIFQL DERRRDAVIA LGIFLIESDL QHKDCVVPYL

    LRLLKGLPKV YWVEESTARK GRGALPVAES FSFCLVTLLS DVAYRDPSLR

    DEILEVLLQV LHVLLGMCQA LEIQDKEYLC KYAIPCLIGI SRAFGRYSNM

    EESLLSKLFP KIPPHSLRVL EELEGVRRRS FNDFRSILPS NLLTVCQEGT

    LKRKTSSVSS ISQVSPERGM PPPSSPGGSA FHYFEASCLP DGTALEPEYY

    FSTISSSFSV SPLFNGVTYK EFNIPLEMLR ELLNLVKKIV EEAVLKSLDA

    IVASVMEANP SADLYYTSFS DPLYLTMFKM LRDTLYYMKD LPTSFVKEIH

    DFVLEQFNTS QGELQKILHD ADRIHNELSP LKLRCQANAA CVDLMVWAVK

    DEQGAENLCI KLSEKLQSKT SSKVIIAHLP LLICCLQGLG RLCERFPVVV

    HSVTPSLRDF LVIPSPVLVK LYKYHSQYHT VAGNDIKISV TNEHSESTLN

    VMSGKKSQPS MYEQLRDIAI DNICRCLKAG LTVDPVIVEA FLASLSNRLY

    ISQESDKDAH LIPDHTIRAL GHIAVALRDT PKVMEPILQI LQQKFCQPPS

    PLDVLIIDQL GCLVITGNQY IYQEVWNLFQ QISVKASSVV YSATKDYKDH

    GYRHCSLAVI NALANIAANI QDEHLVDELL MNLLELFVQL GLEGKRASER

    ASEKGPALKA SSSAGNLGVL IPVIAVLTRR LPPIKEAKPR LQKLFRDFWL

    YSVLMGFAVE GSGLWPEEWY EGVCEIATKS PLLTFPSKEP LRSVLQYNSA

    MKNDTVTPAE LSELRSTIIN LLDPPPEVSA LINKLDFAMS TYLLSVYRLE

    YMRVLRSTDP DRFQVMFCYF EDKAIQKDKS GMMQCVIAVA DKVFDAFLNM

    MADKAKTKEN EEELERHAQF LLVNFNHIHK RIRRVADKYL SGLVDKFPHL

    LWSGTVLKTM LDILQTLSLS LSADIHKDQP YYDIPDAPYR ITVPDTYEAR

    ESIVKDFAAR CGMILQEAMK WAPTVTKSHL QEYLNKHQNW VSGLSQHTGL

    AMATESILHF AGYNKQNTTL GATQLSERPA CVKKDYSNFM ASLNLRNRYA

    GEVYGMIRFS GTTGQMSDLN KMMVQDLHSA LDRSHPQHYT QAMFKLTAML

    ISSKDCDPQL LHHLCWGPLR MFNEHGMETA LACWEWLLAG KDGVEVPFMR

    EMAGAWHMTV EQKFGLFSAE IKEADPLAAS EASQPKPCPP EVTPHYIWID

    FLVQRFEIAK YCSSDQVEIF SSLLQRSMSL NIGGAKGSMN RHVAAIGPRF

    KLLTLGLSLL HADVVPNATI RNVLREKIYS TAFDYFSCPP KFPTQGEKRL

    REDISIMIKF WTAMFSDKKY LTASQLVPPD NQDTRSNLDI TVGSRQQATQ

    GWINTYPLSS GMSTISKKSG MSKKTNRGSQ LHKYYMKRRT LLLSLLATEI

    ERLITWYNPL SAPELELDQA GENSVANWRS KYISLSEKQW KDNVNLAWSI

    SPYLAVQLPA RFKNTEAIGN EVTRLVRLDP GAVSDVPEAI KFLVTWHTID

    ADAPELSHVL CWAPTDPPTG LSYFSSMYPP HPLTAQYGVK VLRSFPPDAI

    LFYIPQIVQA LRYDKMGYVR EYILWAASKS QLLAHQFIWN MKTNIYLDEE

    GHQKDPDIGD LLDQLVEEIT GSLSGPAKDF YQREFDFFNK ITNVSAIIKP

    YPKGDERKKA CLSALSEVKV QPGCYLPSNP EAIVLDIDYK SGTPMQSAAK

    APYLAKFKVK RCGVSELEKE GLRCRSDSED ECSTQEADGQ KISWQAAIFK

    VGDDCRQDML ALQIIDLFKN IFQLVGLDLF VFPYRVVATA PGCGVIECIP

    DCTSRDQLGR QTDFGMYDYF TRQYGDESTL AFQQARYNFI RSMAAYSLLL

    FLLQIKDRHN GNIMLDKKGH IIHIDFGFMF ESSPGGNLGW EPDIKLTDEM

    VMIMGGKMEA TPFKWFMEMC VRGYLAVRPY MDAVVSLVTL MLDTGLPCFR

    GQTIKLLKHR FSPNMTEREA ANFIMKVIQS CFLSNRSRTY DMIQYYQNDI

    PY




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