Mouse VGCC ELISA Kit

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  • Alternative name

    Voltage-dependent L-type calcium channel subunit alpha-1C ELISA KIT; Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle ELISA KIT; Voltage-gated calcium channel subunit alpha Cav1.2CACNA1C ELISA KIT; CACH2 ELISA KIT; CACN2 ELISA KIT; CACNL1A1 ELISA KIT; CCHL1A1 ELISA KIT

  • Catalog
    E072605
  • species
    Mouse
  • GeneVGCC
  • Standard CurveMouse VGCC ELISA Kit
  • Other Species Human VGCC ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse VGCC ELISA Kit allows for the in vitro quantitative determination of VGCC , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate INTENDED USE This VGCC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse VGCC. This ELISA kit for research use only, not for therapeutic or diagnostic applications! PRINCIPLE OF THE ASSAY VGCC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-VGCC antibody and an VGCC-HRP conjugate. The assay sample and buffer are incubated together with VGCC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the VGCC concentration since VGCC from samples and VGCC-HRP conjugate compete for the anti-VGCC antibody binding site. Since the number of sites is limited, as more sites are occupied by VGCC from the sample, fewer sites are left to bind VGCC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VGCC concentration in each sample is interpolated from this standard curve.



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