Human HSP70 ELISA Kit

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  • Alternative name

    Heat shock 70 kDa protein ELISA KIT; HSP70 ELISA KIT; HSP70 ELISA KIT

  • Catalog
  • species
  • GeneHSP70
  • Standard CurveHuman HSP70 ELISA Kit
  • Other Species Mouse HSP70 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Heat Shock Protein 70 (HSP70). No significant cross-reactivity or interference between Heat Shock Protein 70 (HSP70) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 1.25ng/mL.
  • Intended UseHuman HSP70 ELISA Kit allows for the in vitro quantitative determination of HSP70 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 70 (HSP70). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein 70 (HSP70). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein 70 (HSP70), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock Protein 70 (HSP70) in the samples is then determined by comparing the O.D. of the samples to the standard curve.


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