Human ANXA4 ELISA Kit

Alternative name
Human 35-beta calcimedin ELISA Kit;Human Annexin IV ELISA Kit;Human annexin-4 ELISA Kit;Human carbohydrate-binding protein p33/p41 ELISA Kit;Human chromobindin-4 ELISA Kit;Human endonexin I ELISA Kit;Human lipocortin IV ELISA Kit;Human P32.5 ELISA Kit;Human PP4-X ELISA Kit;Human placental anticoagulant protein II ELISA Kit;Human PAP-II ELISA Kit;Human protein II ELISA Kit;Human ANX4 ELISA Kit;Human HEL-S-274 ELISA Kit;Human PIG28 ELISA Kit;Human ZAP36 ELISA Kit;Human annexin A4 ELISA Kit;Human annexin IV (placental anticoagulant protein II) ELISA Kit;Human epididymis secretory protein Li 274 ELISA Kit;Human proliferation-inducing gene 28 ELISA Kit;Human proliferation-inducing protein 28 ELISA Kit;
Catalog
E002417
species
Human
GeneANXA4
Standard Curve
Other Species Mouse Anxa4 ELISA Kit Bovine ANXA4 ELISA Kit Canine ANXA4 ELISA Kit Porcine ANXA4 ELISA Kit Rat Anxa4 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Annexin A4 (ANXA4). No significant cross-reactivity or interference between Annexin A4 (ANXA4) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 7.4pg/mL.
Intended UseHuman ANXA4 ELISA Kit allows for the in vitro quantitative determination of ANXA4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Annexin A4 (ANXA4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Annexin A4 (ANXA4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Annexin A4 (ANXA4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Annexin A4 (ANXA4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Annexin A4 Protein information
Uniprot ID ANXA4_HUMAN
Uniprot AC P09525; B4DDF9; Q96F33; Q9BWK1;
UniGene Hs.422986;
GeneID 307
KEGG hsa:307;
Human Annexin A4 Protein SEQUENCE
SEQUENCE 319 AA; 35883 MW; 3799FE80A93AB215 CRC64;
MATKGGTVKA ASGFNAMEDA QTLRKAMKGL GTDEDAIISV LAYRNTAQRQ
EIRTAYKSTI GRDLIDDLKS ELSGNFEQVI VGMMTPTVLY DVQELRRAMK
GAGTDEGCLI EILASRTPEE IRRISQTYQQ QYGRSLEDDI RSDTSFMFQR
VLVSLSAGGR DEGNYLDDAL VRQDAQDLYE AGEKKWGTDE VKFLTVLCSR
NRNHLLHVFD EYKRISQKDI EQSIKSETSG SFEDALLAIV KCMRNKSAYF
AEKLYKSMKG LGTDDNTLIR VMVSRAEIDM LDIRAHFKRL YGKSLYSFIK
GDTSGDYRKV LLVLCGGDD

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Human ANXA4 ELISA Kit