-
Alternative name
Mitochondrial uncoupling protein 3 ELISA KIT; Solute carrier family 25 member 9Ucp3 ELISA KIT; Slc25a9 ELISA KIT; UCP 3 ELISA KIT
-
Catalog
E071938
-
species
Mouse
-
GeneUCP-3
-
Standard Curve
-
SpecificityThis assay has high sensitivity and excellent specificity for detection ofUCP-3 . No significant cross-reactivity or interference betweenUCP-3 and analogues was observed.
-
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
-
Sensitivity<0.094ng/ml
-
Intended UseMouse UCP-3 ELISA Kit allows for the in vitro quantitative determination of UCP-3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
-
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
-
Product Description
specificalPrinciple of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- UCP-3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-UCP-3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the UCP-3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofUCP-3 can be calculated.
-
Related products with Mouse UCP-3 ELISA Kit