Mouse GRa ELISA Kit

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  • Alternative name

    Glucocorticoid receptor ELISA KIT; Nuclear receptor subfamily 3 group C member 1NR3C1 ELISA KIT; GRL ELISA KIT; GR ELISA KIT

  • Catalog
    E054217
  • species
    Mouse
  • GeneGRa
  • Standard CurveMouse GRa ELISA Kit
  • Other Species Human GRa ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of GRa. No significant cross-reactivity or interference between GRa and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.121ng/mL.
  • Intended UseMouse GRa ELISA Kit allows for the in vitro quantitative determination of GRa , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GRa in mouse tissue homogenates, cell lysates and other biological fluids. Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to GRa. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to GRa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GRa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GRa in the samples is then determined by comparing the O.D. of the samples to the standard curve.


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