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Human MMP9 ELISA Kit

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  • Alternative name

    Human MMP-9 ELISA Kit;Human 92 kDa gelatinase ELISA Kit;Human 92 kDa type IV collagenase ELISA Kit;Human Gelatinase B ELISA Kit;Human GELB ELISA Kit;Human CLG4B ELISA Kit;Human MANDP2 ELISA Kit;Human matrix metallopeptidase 9 ELISA Kit;Human matrix metalloproteinase-9 ELISA Kit;Human macrophage gelatinase ELISA Kit;Human matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) ELISA Kit;Human matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) ELISA Kit;Human type V collagenase ELISA Kit;
  • Catalog
    E023754
  • species
    Human
  • GeneMMP9
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human MMP9. No significant cross-reactivity or interference between Human MMP9 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.284 ng/ml
  • Intended UseHuman MMP9 ELISA Kit allows for the in vitro quantitative determination of MMP9 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Introduction: Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase), also known as MMP9, is an enzyme which in humans is encoded by the MMP9 gene. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Thrombospondins, intervertebral disc proteins, regulate the effective levels of matrix metalloproteinases (MMPs) 2 and 9, which are key effectors of ECM remodeling. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MMP-9. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for MMP-9 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain MMP-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of MMP-9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human 82 kDa matrix metalloproteinase-9 Protein information
  • Uniprot ID MMP9_HUMAN
  • Uniprot AC TTDGRSDG YRWCATTANY DRDKLFGFCP TRADSTVMGG NSAGELCVFP FTFLGKEYST
  • UniGene Hs.297413;
  • GeneID 4318
  • KEGG hsa:4318;
  • Human 82 kDa matrix metalloproteinase-9 Protein SEQUENCE

  • SEQUENCE 707 AA; 78458 MW; 2165AC8CA1466209 CRC64;

    MSLWQPLVLV LLVLGCCFAA PRQRQSTLVL FPGDLRTNLT DRQLAEEYLY

    RYGYTRVAEM RGESKSLGPA LLLLQKQLSL PETGELDSAT LKAMRTPRCG

    VPDLGRFQTF EGDLKWHHHN ITYWIQNYSE DLPRAVIDDA FARAFALWSA

    VTPLTFTRVY SRDADIVIQF GVAEHGDGYP FDGKDGLLAH AFPPGPGIQG

    DAHFDDDELW SLGKGVVVPT RFGNADGAAC HFPFIFEGRS YSACTTDGRS

    DGLPWCSTTA NYDTDDRFGF CPSERLYTQD GNADGKPCQF PFIFQGQSYS

    ACTTDGRSDG YRWCATTANY DRDKLFGFCP TRADSTVMGG NSAGELCVFP

    FTFLGKEYST CTSEGRGDGR LWCATTSNFD SDKKWGFCPD QGYSLFLVAA

    HEFGHALGLD HSSVPEALMY PMYRFTEGPP LHKDDVNGIR HLYGPRPEPE

    PRPPTTTTPQ PTAPPTVCPT GPPTVHPSER PTAGPTGPPS AGPTGPPTAG

    PSTATTVPLS PVDDACNVNI FDAIAEIGNQ LYLFKDGKYW RFSEGRGSRP

    QGPFLIADKW PALPRKLDSV FEERLSKKLF FFSGRQVWVY TGASVLGPRR

    LDKLGLGADV AQVTGALRSG RGKMLLFSGR RLWRFDVKAQ MVDPRSASEV

    DRMFPGVPLD THDVFQYREK AYFCQDRFYW RVSSRSELNQ VDQVGYVTYD

    ILQCPED

  • UCSC uc002xqz.3; human.;



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