Mouse Tnfrsf1a ELISA Kit

Alternative name
Mouse tumor necrosis factor receptor 1 ELISA Kit;Mouse TNF-R1 ELISA Kit;Mouse tumor necrosis factor receptor type I ELISA Kit;Mouse TNF-RI ELISA Kit;Mouse TNFR-I ELISA Kit;Mouse p55 ELISA Kit;Mouse p60 ELISA Kit;Mouse Tnfr-1 ELISA Kit;Mouse Tnfr1 ELISA Kit;Mouse CD120a ELISA Kit;Mouse FPF ELISA Kit;Mouse TNF-R ELISA Kit;Mouse TNF-R-I ELISA Kit;Mouse TNF-R55 ELISA Kit;Mouse TNF-alphaR1 ELISA Kit;Mouse TNFAR ELISA Kit;Mouse TNFR60 ELISA Kit;Mouse TNFRI ELISA Kit;Mouse TNFRp55 ELISA Kit;Mouse TNFalpha-R1 ELISA Kit;Mouse Tnfr-2 ELISA Kit;Mouse p55-R ELISA Kit;Mouse tumor necrosis factor receptor superfamily, member 1a ELISA Kit;Mouse tumor necrosis factor receptor superfamily member 1A ELISA Kit;Mouse TNF receptor alpha chain ELISA Kit;Mouse TNF-alpha-R1 ELISA Kit;
Catalog
E070320
species
Mouse
GeneTnfrsf1a
SpecificityNatural and recombinant mouse TNFR1
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity Sample For quantitative detection of mouse TNFR1 in cell culture supernates and serum.
Intended UseMouse Tnfrsf1a ELISA Kit allows for the in vitro quantitative determination of Tnfrsf1a , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: Mouse TNFR1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFR1 has been precoated onto 96-well plates. Standards (NSO, L30-A212) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFR1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFR1 amount of sample captured in plate. Background: Tumor necrosis factor receptor 1 (TNFR1), also known as tumor necrosis factor receptor superfamily member 1A (TNFRSF1A) and CD120a, is a ubiquitous membrane receptor that binds tumor necrosis factor-alpha (TNF alpha). The protein encoded by this gene is a member of the tumor necrosis factor receptor superfamily, which also contains TNFRSF1B. And this protein is one of the major receptors for the tumor necrosis factor-alpha. This receptor can activate the transcription factor NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor.
Mouse Tumor necrosis factor receptor superfamily member 1A Protein information
Uniprot ID TNR1A_MOUSE
Uniprot AC P25118;
UniGene Mm.1258; Mm.474976;
GeneID 21937
KEGG mmu:21937;
Mouse Tumor necrosis factor receptor superfamily member 1A Protein SEQUENCE
SEQUENCE 454 AA; 50130 MW; 0710C2E8C3C2B6D9 CRC64;
MGLPTVPGLL LSLVLLALLM GIHPSGVTGL VPSLGDREKR DSLCPQGKYV
HSKNNSICCT KCHKGTYLVS DCPSPGRDTV CRECEKGTFT ASQNYLRQCL
SCKTCRKEMS QVEISPCQAD KDTVCGCKEN QFQRYLSETH FQCVDCSPCF
NGTVTIPCKE TQNTVCNCHA GFFLRESECV PCSHCKKNEE CMKLCLPPPL
ANVTNPQDSG TAVLLPLVIL LGLCLLSFIF ISLMCRYPRW RPEVYSIICR
DPVPVKEEKA GKPLTPAPSP AFSPTSGFNP TLGFSTPGFS SPVSSTPISP
IFGPSNWHFM PPVSEVVPTQ GADPLLYESL CSVPAPTSVQ KWEDSAHPQR
PDNADLAILY AVVDGVPPAR WKEFMRFMGL SEHEIERLEM QNGRCLREAQ
YSMLEAWRRR TPRHEDTLEV VGLVLSKMNL AGCLENILEA LRNPAPSSTT
RLPR
UCSC uc009dul.2; mouse.;

Category

Mouse Tnfrsf1a ELISA Kit