Mouse Sf1 ELISA Kit

Alternative name
Mouse CW17 ELISA Kit;Mouse Mammalian branch point-binding protein ELISA Kit;Mouse BBP ELISA Kit;Mouse mBBP ELISA Kit;Mouse Transcription factor ZFM1 ELISA Kit;Mouse mZFM ELISA Kit;Mouse Zinc finger gene in MEN1 locus ELISA Kit;Mouse Zinc finger protein 162 ELISA Kit;Mouse Zfm1 ELISA Kit;Mouse Zfp162 ELISA Kit;
Catalog
E067614
species
Mouse
GeneSf1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse SF1. No significant cross-reactivity or interference between Mouse SF1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse SF1 and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseMouse Sf1 ELISA Kit allows for the in vitro quantitative determination of Sf1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SF1 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse SF1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse SF1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SF1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse SF1. You can calculate the concentration of Mouse SF1 in the samples by comparing the OD of the samples to the standard curve.
Mouse Splicing factor 1 Protein information
Uniprot ID SF01_MOUSE
Uniprot AC Q64213; E9QK02; O08817; P70167; Q61454; Q921Z4;
UniGene Mm.256422; Mm.453047;
Mouse Splicing factor 1 Protein SEQUENCE
SEQUENCE 653 AA; 70403 MW; 5C159302822509D1 CRC64;
MATGANATPL DFPSKKRKRS RWNQDTMEQK TVIPGMPTVI PPGLTREQER
AYIVQLQIED LTRKLRTGDL GIPPNPEDRS PSPEPIYNSE GKRLNTREFR
TRKKLEEERH TLITEMVALN PDFKPPADYK PPATRVSDKV MIPQDEYPEI
NFVGLLIGPR GNTLKNIEKE CNAKIMIRGK GSVKEGKVGR KDGQMLPGED
EPLHALVTAN TMENVKKAVE QIRNILKQGI ETPEDQNDLR KMQLRELARL
NGTLREDDNR ILRPWQSSET RSITNTTVCT KCGGAGHIAS DCKFQRPGDP
QSAQDKARMD KEYLSLMAEL GEAPVPASVG STSGPATTPL ASAPRPAAPA
SNPPPPSLMS TTQSRPPWMN SGPSENRPYH GMHGGGPGGP GGGPHSFPHP
LPSLTGGHGG HPMQHNPNGP PPPWMQPPPP PMNQGPHPPG HHGPPPMDQY
LGSTPVGSGV YRLHQGKGMM PPPPMGMMPP PPPPPSGQPP PPPSGPLPPW
QQQQQQPPPP PPPSSSMASS TPLPWQQNTT TTTTSAGTGS IPPWQQQQAA
AAASPGTPQM QGNPTMVPLP PGVQPPLPPG APPPPPCSIE CLLCLLSSPN
SLCLSPNRAA RIPPRGSDGP SHESEDFPRP LVTLPGRQPQ QRPWWTGWFG
KAA

Category

Mouse Sf1 ELISA Kit