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Human NODAL ELISA Kit

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  • Alternative name

    Human HTX5 ELISA Kit;Human nodal growth differentiation factor ELISA Kit;Human nodal homolog ELISA Kit;
  • Catalog
    E026693
  • species
    Human
  • GeneNODAL
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human NODAL. No significant cross-reactivity or interference between human NODAL and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human NODAL is typically less than 19.5 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
  • Intended UseHuman NODAL ELISA Kit allows for the in vitro quantitative determination of NODAL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NODAL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NODAL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NODAL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NODAL bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Nodal homolog Protein information
  • Uniprot ID NODAL_HUMAN
  • Uniprot AC Q96S42; Q2M3A5; Q8N4V3;
  • UniGene Hs.370414;
  • GeneID 4838
  • KEGG hsa:4838;
  • Human Nodal homolog Protein SEQUENCE

  • SEQUENCE 347 AA; 39561 MW; 0D52352B9711650C CRC64;

    MHAHCLPFLL HAWWALLQAG AATVATALLR TRGQPSSPSP LAYMLSLYRD

    PLPRADIIRS LQAEDVAVDG QNWTFAFDFS FLSQQEDLAW AELRLQLSSP

    VDLPTEGSLA IEIFHQPKPD TEQASDSCLE RFQMDLFTVT LSQVTFSLGS

    MVLEVTRPLS KWLKHPGALE KQMSRVAGEC WPRPPTPPAT NVLLMLYSNL

    SQEQRQLGGS TLLWEAESSW RAQEGQLSWE WGKRHRRHHL PDRSQLCRKV

    KFQVDFNLIG WGSWIIYPKQ YNAYRCEGEC PNPVGEEFHP TNHAYIQSLL

    KRYQPHRVPS TCCAPVKTKP LSMLYVDNGR VLLDHHKDMI VEECGCL

  • UCSC uc001jrc.3; human.;



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