Human C1q ELISA Kit

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  • Alternative name

    Complement C1q tumor necrosis factor-related protein 5 ELISA KIT; C1QTNF5 ELISA KIT; CTRP5 ELISA KIT; UNQ303/PRO344 ELISA KIT

  • Catalog
    E002540
  • species
    Human
  • GeneC1q
  • Standard CurveHuman C1q ELISA Kit
  • Other Species Human C1q-Ab ELISA KitMouse C1Q ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman C1q ELISA Kit allows for the in vitro quantitative determination of C1q , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyImmunology
  • Product Description
    specifical
    For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate INTENDED USE This Anti-C1q ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human Anti-C1q. This ELISA kit for research use only, not for therapeutic or diagnostic applications! PRINCIPLE OF THE ASSAY Anti-C1q ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Anti-C1q antibody and an Anti-C1q-HRP conjugate. The assay sample and buffer are incubated together with Anti-C1q-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Anti-C1q concentration since Anti-C1q from samples and Anti-C1q-HRP conjugate compete for the anti-Anti-C1q antibody binding site. Since the number of sites is limited, as more sites are occupied by Anti-C1q from the sample, fewer sites are left to bind Anti-C1q-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-C1q concentration in each sample is interpolated from this standard curve.


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