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Alternative name
Carnosine synthase 1 ELISA KIT; ATP-grasp domain-containing protein 1Carns1 ELISA KIT; Atpgd1 ELISA KIT; Kiaa1394 ELISA KIT
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Catalog
E006757
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species
Human
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GeneATPGD1
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SpecificityThis assay has high sensitivity and excellent specificity for detection of CARNS1. No significant cross-reactivity or interference between CARNS1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CARNS1 and all the analogues, therefore, cross reaction may still exist in some cases.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.1 ng/mL.
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Intended UseHuman ATPGD1 ELISA Kit allows for the in vitro quantitative determination of ATPGD1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilySignal Transduction
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Product Description
specificalIntended Uses: This CARNS1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CARNS1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay||CARNS1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CARNS1 antibody and an CARNS1-HRP conjugate. The assay sample and buffer are incubated together with CARNS1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CARNS1 concentration since CARNS1 from samples and CARNS1-HRP conjugate compete for the anti-CARNS1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CARNS1 from the sample, fewer sites are left to bind CARNS1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CARNS1 concentration in each sample is interpolated from this standard curve.
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