SensitivityThe minimum detectable dose of human 5- HETE is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the
Intended UseHuman 5-HETE ELISA Kit allows for the in vitro quantitative determination of 5-HETE , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for 5-HETE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any 5-HETE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 5-HETE is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 5-HETE bound in the initial step. The color development is stopped and the intensity of the color is measured.