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Mouse beta-APP ELISA Kit

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  • Alternative name

    Amyloid beta A4 protein ELISA KIT; ABPP ELISA KIT; APPI ELISA KIT; APP ELISA KIT; Alzheimer disease amyloid protein ELISA KIT; Cerebral vascular amyloid peptide ELISA KIT; CVAP ELISA KIT; PreA4 ELISA KIT; Protease nexin-II ELISA KIT; PN-IIAPP ELISA KIT; A4 ELISA KIT; AD1 ELISA KIT; APP ELISA KIT; CVAP ELISA KIT; PN-II ELISA KIT; S-APP-alpha ELISA KIT; S-APP-beta ELISA KIT; AICD-59 ELISA KIT; AID(59) ELISA KIT; AICD-57 ELISA KIT; AID(57) ELISA KIT; AICD-50 ELISA KIT; AID(50) ELISA KIT
  • Catalog
    E045588
  • species
    Mouse
  • Genebeta-APP
  • Standard CurveMouse beta-APP ELISA Kit
  • Other Species Human beta APP ELISA KitHuman beta-APP ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse beta-APP ELISA Kit allows for the in vitro quantitative determination of beta-APP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyNeurobiology
  • Product Description
    specifical    Principle of the Assay: beta-APP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-beta-APP antibody and an beta-APP-HRP conjugate. The assay sample and buffer are incubated together with beta-APP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the beta-APP concentration since beta-APP from samples and beta-APP-HRP conjugate compete for the anti-beta-APP antibody binding site. Since the number of sites is limited, as more sites are occupied by beta-APP from the sample, fewer sites are left to bind beta-APP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The beta-APP concentration in each sample is interpolated from this standard curve.


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