Mouse Serpina3n ELISA Kit

Alternative name
Mouse Serpin A3N ELISA Kit;Mouse Spi2 ELISA Kit;
Catalog
E067366
species
Mouse
GeneSerpina3n
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.094ng/ml
Intended UseMouse Serpina3n ELISA Kit allows for the in vitro quantitative determination of Serpina3n , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Serpina3n antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Serpina3n antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Serpina3n amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofMouse Serpina3n can be calculated.
Mouse Serine protease inhibitor A3N Protein information
Uniprot ID SPA3N_MOUSE
Uniprot AC Q91WP6; Q62258;
UniGene Mm.482074;
Mouse Serine protease inhibitor A3N Protein SEQUENCE
SEQUENCE 418 AA; 46718 MW; 4BF21BBE10A81F6C CRC64;
MAFIAALGLL MAGICPAVLC FPDGTLGMDA AVQEDHDNGT QLDSLTLASI
NTDFAFSLYK ELVLKNPDKN IVFSPLSISA ALAVMSLGAK GNTLEEILEG
LKFNLTETSE ADIHQGFGHL LQRLNQPKDQ VQISTGSALF IEKRQQILTE
FQEKAKTLYQ AEAFTADFQQ PRQAKKLIND YVRKQTQGMI KELVSDLDKR
TLMVLVNYIY FKAKWKVPFD PLDTFKSEFY AGKRRPVIVP MMSMEDLTTP
YFRDEELSCT VVELKYTGNA SALFILPDQG RMQQVEASLQ PETLRKWKNS
LKPRMIDELH LPKFSISTDY SLEDVLSKLG IREVFSTQAD LSAITGTKDL
RVSQVVHKAV LDVAETGTEA AAATGVKFVP MSAKLYPLTV YFNRPFLIMI
FDTETEIAPF IAKIANPK

Category

Mouse Serpina3n ELISA Kit

Mouse Serpin A3N ELISA Kit;Mouse Spi2 ELISA Kit;