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Human FXN ELISA Kit

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  • Alternative name

    Human Friedreich ataxia protein ELISA Kit;Human Fxn ELISA Kit;Human i-FXN ELISA Kit;Human m56-FXN ELISA Kit;Human d-FXN ELISA Kit;Human m78-FXN ELISA Kit;Human Frataxin(81-210) ELISA Kit;Human m81-FXN ELISA Kit;Human FRDA ELISA Kit;Human X25 ELISA Kit;Human CyaY ELISA Kit;Human FA ELISA Kit;Human FARR ELISA Kit;Human frataxin ELISA Kit;Human frataxin, mitochondrial ELISA Kit;
  • Catalog
    E015504
  • species
    Human
  • GeneFXN
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human FXN. No significant cross-reactivity or interference between human FXN and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human FXN is typically less than 5.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
  • Intended UseHuman FXN ELISA Kit allows for the in vitro quantitative determination of FXN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FXN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FXN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FXN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FXN bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Frataxin mature form Protein information
  • Uniprot ID FRDA_HUMAN
  • Uniprot AC Q16595; A8MXJ6; C9JJ89; O15545; O95656; Q15294; Q5VZ01;
  • UniGene Hs.20685;
  • GeneID 2395
  • KEGG hsa:2395;
  • Human Frataxin mature form Protein SEQUENCE

  • SEQUENCE 210 AA; 23135 MW; ECC81738779308CF CRC64;

    MWTLGRRAVA GLLASPSPAQ AQTLTRVPRP AELAPLCGRR GLRTDIDATC

    TPRRASSNQR GLNQIWNVKK QSVYLMNLRK SGTLGHPGSL DETTYERLAE

    ETLDSLAEFF EDLADKPYTF EDYDVSFGSG VLTVKLGGDL GTYVINKQTP

    NKQIWLSSPS SGPKRYDWTG KNWVYSHDGV SLHELLAAEL TKALKTKLDL

    SSLAYSGKDA

  • UCSC uc004agz.3; human. [Q16595-1];



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