Human NONO ELISA Kit

Alternative name
Human NonO protein ELISA Kit;Human 54 kDa nuclear RNA- and DNA-binding protein ELISA Kit;Human 55 kDa nuclear protein ELISA Kit;Human DNA-binding p52/p100 complex, 52 kDa subunit ELISA Kit;Human NMT55 ELISA Kit;Human p54(nrb) ELISA Kit;Human P54NRB ELISA Kit;Human NRB54 ELISA Kit;Human MRXS34 ELISA Kit;Human P54 ELISA Kit;Human PPP1R114 ELISA Kit;Human non-POU domain containing, octamer-binding ELISA Kit;Human non-POU domain-containing octamer-binding protein ELISA Kit;Human non-POU domain-containing octamer (ATGCAAAT) binding protein ELISA Kit;Human protein phosphatase 1, regulatory subunit 114 ELISA Kit;
Catalog
E026717
species
Human
GeneNONO
SpecificityThis assay has high sensitivity and excellent specificity for detection of Non POU Domain Containing Octamer Binding Protein (NONO). No significant cross-reactivity or interference between Non POU Domain Containing Octamer Binding Protein (NONO) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.056ng/mL.
Intended UseHuman NONO ELISA Kit allows for the in vitro quantitative determination of NONO , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Non POU Domain Containing Octamer Binding Protein (NONO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Non POU Domain Containing Octamer Binding Protein (NONO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Non POU Domain Containing Octamer Binding Protein (NONO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Non POU Domain Containing Octamer Binding Protein (NONO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Non-POU domain-containing octamer-binding protein Protein information
Uniprot ID NONO_HUMAN
Uniprot AC Q15233; B7Z4C2; D3DVV4; F5GYZ3; O00201; P30807; Q12786; Q9BQC5;
UniGene Hs.533282; Hs.700344;
GeneID 4841
KEGG hsa:4841;
Human Non-POU domain-containing octamer-binding protein Protein SEQUENCE
SEQUENCE 471 AA; 54232 MW; 26BBD3828F5B9E49 CRC64;
MQSNKTFNLE KQNHTPRKHH QHHHQQQHHQ QQQQQPPPPP IPANGQQASS
QNEGLTIDLK NFRKPGEKTF TQRSRLFVGN LPPDITEEEM RKLFEKYGKA
GEVFIHKDKG FGFIRLETRT LAEIAKVELD NMPLRGKQLR VRFACHSASL
TVRNLPQYVS NELLEEAFSV FGQVERAVVI VDDRGRPSGK GIVEFSGKPA
ARKALDRCSE GSFLLTTFPR PVTVEPMDQL DDEEGLPEKL VIKNQQFHKE
REQPPRFAQP GSFEYEYAMR WKALIEMEKQ QQDQVDRNIK EAREKLEMEM
EAARHEHQVM LMRQDLMRRQ EELRRMEELH NQEVQKRKQL ELRQEEERRR
REEEMRRQQE EMMRRQQEGF KGTFPDAREQ EIRMGQMAMG GAMGINNRGA
MPPAPVPAGT PAPPGPATMM PDGTLGLTPP TTERFGQAAT MEGIGAIGGT
PPAFNRAAPG AEFAPNKRRR Y
UCSC uc004dzn.5; human. [Q15233-1];

Category

Human NONO ELISA Kit