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Alternative name
Mouse EG433180 ELISA Kit;Mouse serine peptidase inhibitor, Kazal type 6 ELISA Kit;Mouse serine protease inhibitor Kazal-type 6 ELISA Kit;
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Catalog
E067349
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species
Mouse
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GeneSpink6
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SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse SPINK6. No significant cross-reactivity or interference between mouse SPINK6 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of mouse SPINK6 is typically less than 0.039ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the m
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Intended UseMouse Spink6 ELISA Kit allows for the in vitro quantitative determination of Spink6 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SPINK6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPINK6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPINK6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPINK6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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Mouse Serine protease inhibitor Kazal-type 6 Protein information
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Uniprot ID
ISK6_MOUSE
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Uniprot AC
Q8BT20;
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UniGene
Mm.57302;
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GeneID
433180
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KEGG
mmu:433180;
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Mouse Serine protease inhibitor Kazal-type 6 Protein SEQUENCE
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SEQUENCE 105 AA; 11609 MW; CB59A06715C2C589 CRC64;
MKVAGVFLLL SLALLCFFSG AFSQGGQDKR GWITRSEGRF PGKGVLRHRL
FQINCGEFRD
PKVFCTRESD PLCGSDGQTY GNKCAFCKAL EKSSGKINLK
HRGKC
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UCSC
uc008eur.1; mouse.;
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