Human DEFB112 ELISA Kit

Alternative name
Human beta-defensin 12 ELISA Kit;Human DEFB-12 ELISA Kit;Human Defensin, beta 112 ELISA Kit;Human DEFB12 ELISA Kit;Human defensin beta 112 ELISA Kit;Human beta-defensin 112 ELISA Kit;Human defensin, beta 12 ELISA Kit;
Catalog
E011502
species
Human
GeneDEFB112
SpecificityThis assay has high sensitivity and excellent specificity for detection of Defensin Beta 112 (DEFb112). No significant cross-reactivity or interference between Defensin Beta 112 (DEFb112) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 29.4pg/mL.
Intended UseHuman DEFB112 ELISA Kit allows for the in vitro quantitative determination of DEFB112 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 112 (DEFb112). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Beta 112 (DEFb112). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 112 (DEFb112), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Defensin Beta 112 (DEFb112) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Beta-defensin 112 Protein information
Uniprot ID DB112_HUMAN
Uniprot AC Q30KQ8; Q8NET0;
UniGene Hs.571092;
GeneID 245915
KEGG hsa:245915;
Human Beta-defensin 112 Protein SEQUENCE
SEQUENCE 113 AA; 12991 MW; 53617991C2160524 CRC64;
MKLLTTICRL KLEKMYSKTN TSSTIFEKAR HGTEKISTAR SEGHHITFSR
WKSCTAIGGR CKNQCDDSEF RISYCARPTT HCCVTECDPT DPNNWIPKDS
VGTQEWYPKD SRH
UCSC uc011dws.2; human.;

Human DEFB112 ELISA Kit

Human beta-defensin 12 ELISA Kit;Human DEFB-12 ELISA Kit;Human Defensin, beta 112 ELISA Kit;Human DEFB12 ELISA Kit;Human defensin beta 112 ELISA Kit;Human beta-defensin 112 ELISA Kit;Human defensin, beta 12 ELISA Kit;

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