SpecificityThis assay has high sensitivity and excellent specificity for detection of Defensin Beta 112 (DEFb112).
No significant cross-reactivity or interference between Defensin Beta 112 (DEFb112) and analogues was observed.
Intended UseHuman DEFB112 ELISA Kit allows for the in vitro quantitative determination of DEFB112 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 112 (DEFb112). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Beta 112 (DEFb112). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 112 (DEFb112), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Defensin Beta 112 (DEFb112) in the samples is then determined by comparing the O.D. of the samples to the standard curve.