Human CRB1 ELISA Kit

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  • Alternative name

    Human LCA8 ELISA Kit;Human RP12 ELISA Kit;Human crumbs 1, cell polarity complex component ELISA Kit;Human protein crumbs homolog 1 ELISA Kit;Human crumbs family member 1, photoreceptor morphogenesis associated ELISA Kit;

  • Catalog
    E010262
  • species
    Human
  • GeneCRB1
  • Standard CurveHuman CRB1 ELISA Kit
  • Other Species Mouse Crb1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CRB1. No significant cross-reactivity or interference between CRB1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CRB1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CRB1 ELISA Kit allows for the in vitro quantitative determination of CRB1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyNeurobiology
  • Product Description
    specifical
    Principle of the assay: CRB1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CRB1 antibody and an CRB1-HRP conjugate. The assay sample and buffer are incubated together with CRB1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CRB1 concentration since CRB1 from samples and CRB1-HRP conjugate compete for the anti-CRB1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CRB1 from the sample, fewer sites are left to bind CRB1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CRB1 concentration in each sample is interpolated from this standard curve.
  • Human Protein crumbs homolog 1 Protein information
  • Uniprot ID CRUM1_HUMAN
  • Uniprot AC Q8WWY0; Q8WWY1;
  • UniGene Hs.126135;
  • GeneID 23418
  • KEGG hsa:23418;
  • Human Protein crumbs homolog 1 Protein SEQUENCE
  • SEQUENCE 1406 AA; 154183 MW; F2D04D20FAA6E37D CRC64;

    MALKNINYLL IFYLSFSLLI YIKNSFCNKN NTRCLSNSCQ NNSTCKDFSK

    DNDCSCSDTA NNLDKDCDNM KDPCFSNPCQ GSATCVNTPG ERSFLCKCPP

    GYSGTICETT IGSCGKNSCQ HGGICHQDPI YPVCICPAGY AGRFCEIDHD

    ECASSPCQNG AVCQDGIDGY SCFCVPGYQG RHCDLEVDEC ASDPCKNEAT

    CLNEIGRYTC ICPHNYSGVN CELEIDECWS QPCLNGATCQ DALGAYFCDC

    APGFLGDHCE LNTDECASQP CLHGGLCVDG ENRYSCNCTG SGFTGTHCET

    LMPLCWSKPC HNNATCEDSV DNYTCHCWPG YTGAQCEIDL NECNSNPCQS

    NGECVELSSE KQYGRITGLP SSFSYHEASG YVCICQPGFT GIHCEEDVNE

    CSSNPCQNGG TCENLPGNYT CHCPFDNLSR TFYGGRDCSD ILLGCTHQQC

    LNNGTCIPHF QDGQHGFSCL CPSGYTGSLC EIATTLSFEG DGFLWVKSGS

    VTTKGSVCNI ALRFQTVQPM ALLLFRSNRD VFVKLELLSG YIHLSIQVNN

    QSKVLLFISH NTSDGEWHFV EVIFAEAVTL TLIDDSCKEK CIAKAPTPLE

    SDQSICAFQN SFLGGLPVGM TSNGVALLNF YNMPSTPSFV GCLQDIKIDW

    NHITLENISS GSSLNVKAGC VRKDWCESQP CQSRGRCINL WLSYQCDCHR

    PYEGPNCLRE YVAGRFGQDD STGYVIFTLD ESYGDTISLS MFVRTLQPSG

    LLLALENSTY QYIRVWLERG RLAMLTPNSP KLVVKFVLND GNVHLISLKI

    KPYKIELYQS SQNLGFISAS TWKIEKGDVI YIGGLPDKQE TELNGGFFKG

    CIQDVRLNNQ NLEFFPNPTN NASLNPVLVN VTQGCAGDNS CKSNPCHNGG

    VCHSRWDDFS CSCPALTSGK ACEEVQWCGF SPCPHGAQCQ PVLQGFECIA

    NAVFNGQSGQ ILFRSNGNIT RELTNITFGF RTRDANVIIL HAEKEPEFLN

    ISIQDSRLFF QLQSGNSFYM LSLTSLQSVN DGTWHEVTLS MTDPLSQTSR

    WQMEVDNETP FVTSTIATGS LNFLKDNTDI YVGDRAIDNI KGLQGCLSTI

    EIGGIYLSYF ENVHGFINKP QEEQFLKIST NSVVTGCLQL NVCNSNPCLH

    GGNCEDIYSS YHCSCPLGWS GKHCELNIDE CFSNPCIHGN CSDRVAAYHC

    TCEPGYTGVN CEVDIDNCQS HQCANGATCI SHTNGYSCLC FGNFTGKFCR

    QSRLPSTVCG NEKTNLTCYN GGNCTEFQTE LKCMCRPGFT GEWCEKDIDE

    CASDPCVNGG LCQDLLNKFQ CLCDVAFAGE RCEVDLADDL ISDIFTTIGS

    VTVALLLILL LAIVASVVTS NKRATQGTYS PSRQEKEGSR VEMWNLMPPP

    AMERLI

  • UCSC uc001gtz.4; human. [P82279-1];



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