Mouse Retn ELISA Kit

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  • Alternative name

    Mouse adipose tissue-specific secretory factor ELISA Kit;Mouse ADSF ELISA Kit;Mouse adipose-specific cysteine-rich secreted protein A12-alpha ELISA Kit;Mouse cysteine-rich secreted protein FIZZ3 ELISA Kit;Mouse Fizz3 ELISA Kit;Mouse Rstn ELISA Kit;Mouse Xcp4 ELISA Kit;Mouse resistin ELISA Kit;Mouse dominant inhibitory adipocyte-specific secretory factor ELISA Kit;Mouse found in inflammatory zone 3 ELISA Kit;

  • Catalog
    E066255
  • species
    Mouse
  • GeneRetn
  • Standard CurveMouse Retn ELISA Kit
  • Other Species Human RETN ELISA KitBovine RETN ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 2pg/ml
  • Intended UseMouse Retn ELISA Kit allows for the in vitro quantitative determination of Retn , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Resistin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Resistin polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Resistin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Resistin can be calculated. Background: Resistin also known as adipose tissue-specific secretory factor or C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein (XCP1) is a cysteine-rich protein that in humans is encoded by the RETN gene. The gene comprises 4 exons, the first of which is untranslated, and spans approximately 1,750 bp. The length of the resistin pre-peptide in human is 108 amino acid residues and in the mouse and rat it is 114 aa; the molecular weight is ~12.5 kDa. Resistin is a cytokine whose physiologic role has been the subject of much controversy regarding its involvement with obesity and type II diabetes mellitus (T2DM).
  • Mouse Resistin Protein information
  • Uniprot ID RETN_MOUSE
  • Uniprot AC Q99P87;
  • UniGene Mm.1181;
  • GeneID 57264
  • KEGG mmu:57264;
  • Mouse Resistin Protein SEQUENCE
  • SEQUENCE 114 AA; 12492 MW; D44930E51D3F22C8 CRC64;

    MKNLSFPLLF LFFLVPELLG SSMPLCPIDE AIDKKIKQDF NSLFPNAIKN

    IGLNCWTVSS RGKLASCPEG TAVLSCSCGS ACGSWDIREE KVCHCQCARI

    DWTAARCCKL QVAS

  • UCSC uc009ksg.2; mouse.;



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