Human IFI35 ELISA Kit

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  • Alternative name

    Human IFP 35 ELISA Kit;Human ifi-35 ELISA Kit;Human IFP35 ELISA Kit;Human interferon induced protein 35 ELISA Kit;Human interferon-induced 35 kDa protein ELISA Kit;

  • Catalog
    E020311
  • species
    Human
  • GeneIFI35
  • Standard CurveHuman IFI35 ELISA Kit
  • Other Species Mouse Ifi35 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Interferon Inducible Protein 35 (IFI35). No significant cross-reactivity or interference between Interferon Inducible Protein 35 (IFI35) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 31pg/mL.
  • Intended UseHuman IFI35 ELISA Kit allows for the in vitro quantitative determination of IFI35 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Inducible Protein 35 (IFI35). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Inducible Protein 35 (IFI35). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Inducible Protein 35 (IFI35), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interferon Inducible Protein 35 (IFI35) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Interferon-induced 35 kDa protein Protein information
  • Uniprot ID IN35_HUMAN
  • Uniprot AC P80217; C9JGX1; Q92984; Q99537; Q9BV98;
  • UniGene Hs.632258;
  • GeneID 3430
  • KEGG hsa:3430;
  • Human Interferon-induced 35 kDa protein Protein SEQUENCE
  • SEQUENCE 286 AA; 31546 MW; 3FCAB26E97317508 CRC64;

    MSAPLDAALH ALQEEQARLK MRLWDLQQLR KELGDSPKDK VPFSVPKIPL

    VFRGHTQQDP EVPKSLVSNL RIHCPLLAGS ALITFDDPKV AEQVLQQKEH

    TINMEECRLR VQVQPLELPM VTTIQMSSQL SGRRVLVTGF PASLRLSEEE

    LLDKLEIFFG KTRNGGGDVD VRELLPGSVM LGFARDGVAQ RLCQIGQFTV

    PLGGQQVPLR VSPYVNGEIQ KAEIRSQPVP RSVLVLNIPD ILDGPELHDV

    LEIHFQKPTR GGGEVEALTV VPQGQQGLAV FTSESG

  • UCSC uc021txx.2; human. [P80217-1];


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