Mouse PDIA2 ELISA Kit

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  • Alternative name

    Protein disulfide-isomerase A2 ELISA KIT; Pancreas-specific protein disulfide isomerase ELISA KIT; PDIpPDIA2 ELISA KIT; PDIP ELISA KIT; PDIp ELISA KIT

  • Catalog
    E065081
  • species
    Mouse
  • GenePDIA2
  • Standard CurveMouse PDIA2 ELISA Kit
  • Other Species Human PDIA2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Protein Disulfide Isomerase A2 (PDIA2). No significant cross-reactivity or interference between Protein Disulfide Isomerase A2 (PDIA2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.064ng/mL.
  • Intended UseMouse PDIA2 ELISA Kit allows for the in vitro quantitative determination of PDIA2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Disulfide Isomerase A2 (PDIA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Disulfide Isomerase A2 (PDIA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Disulfide Isomerase A2 (PDIA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Disulfide Isomerase A2 (PDIA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.


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