Human ADAM28 ELISA Kit

Alternative name
Human ADAM 28 ELISA Kit;Human epididymal metalloproteinase-like, disintegrin-like, and cysteine-rich protein II ELISA Kit;Human eMDC II ELISA Kit;Human Metalloproteinase-like, disintegrin-like, and cysteine-rich protein L ELISA Kit;Human MDC-L ELISA Kit;Human ADAM23 ELISA Kit;Human MDCL ELISA Kit;Human eMDCII ELISA Kit;Human ADAM metallopeptidase domain 28 ELISA Kit;Human disintegrin and metalloproteinase domain-containing protein 28 ELISA Kit;Human epididymial metalloproteinase-like, disintegrin-like, and cysteine-rich protein II ELISA Kit;Human metalloproteinase-like, disintegrin-like, and cysteine-rich protein-L ELISA Kit;
Catalog
E000663
species
Human
GeneADAM28
Other Species Mouse Adam28 ELISA Kit Monkey ADAM28 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of human ADAM28. No significant cross-reactivity or interference between human ADAM28 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human ADAM28 is typically less than 0.039 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the m
Intended UseHuman ADAM28 ELISA Kit allows for the in vitro quantitative determination of ADAM28 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADAM28 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADAM28 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAM28 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAM28 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Disintegrin and metalloproteinase domain-containing protein 28 Protein information
Uniprot ID ADA28_HUMAN
Uniprot AC Q9UKQ2; B2RMV5; Q9Y339; Q9Y3S0;
UniGene Hs.174030; Hs.388903;
GeneID 10863
KEGG hsa:10863;
Human Disintegrin and metalloproteinase domain-containing protein 28 Protein SEQUENCE
SEQUENCE 775 AA; 87148 MW; 895E0985840F971C CRC64;
MLQGLLPVSL LLSVAVSAIK ELPGVKKYEV VYPIRLHPLH KREAKEPEQQ
EQFETELKYK MTINGKIAVL YLKKNKNLLA PGYTETYYNS TGKEITTSPQ
IMDDCYYQGH ILNEKVSDAS ISTCRGLRGY FSQGDQRYFI EPLSPIHRDG
QEHALFKYNP DEKNYDSTCG MDGVLWAHDL QQNIALPATK LVKLKDRKVQ
EHEKYIEYYL VLDNGEFKRY NENQDEIRKR VFEMANYVNM LYKKLNTHVA
LVGMEIWTDK DKIKITPNAS FTLENFSKWR GSVLSRRKRH DIAQLITATE
LAGTTVGLAF MSTMCSPYSV GVVQDHSDNL LRVAGTMAHE MGHNFGMFHD
DYSCKCPSTI CVMDKALSFY IPTDFSSCSR LSYDKFFEDK LSNCLFNAPL
PTDIISTPIC GNQLVEMGED CDCGTSEECT NICCDAKTCK IKATFQCALG
ECCEKCQFKK AGMVCRPAKD ECDLPEMCNG KSGNCPDDRF QVNGFPCHHG
KGHCLMGTCP TLQEQCTELW GPGTEVADKS CYNRNEGGSK YGYCRRVDDT
LIPCKANDTM CGKLFCQGGS DNLPWKGRIV TFLTCKTFDP EDTSQEIGMV
ANGTKCGDNK VCINAECVDI EKAYKSTNCS SKCKGHAVCD HELQCQCEEG
WIPPDCDDSS VVFHFSIVVG VLFPMAVIFV VVAMVIRHQS SREKQKKDQR
PLSTTGTRPH KQKRKPQMVK AVQPQEMSQM KPHVYDLPVE GNEPPASFHK
DTNALPPTVF KDNPVSTPKD SNPKA
UCSC uc003xdx.4; human. [Q9UKQ2-1];

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Human ADAM28 ELISA Kit